Difference between revisions of "YumC"
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| − | * '''Description:''' ferredoxin-NAD(P)+ oxidoreductase<br/><br/> | + | * '''Description:''' ferredoxin/flavodoxin-NAD(P)+ oxidoreductase<br/><br/> |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32110 yumC] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32110 yumC] | ||
| + | |- | ||
| + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=YumC YumC] | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 5.573 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 5.573 | ||
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' 2 reduced ferredoxin + NADP<sup>+</sup> + H<sup>+</sup> = 2 oxidized ferredoxin + NADPH (according to Swiss-Prot) | + | * '''Catalyzed reaction/ biological activity:''' 2 reduced ferredoxin or flavodoxin + NADP<sup>+</sup> + H<sup>+</sup> = 2 oxidized ferredoxin or flavodoxin + NADPH (according to Swiss-Prot) |
| − | * '''Protein family:''' ferredoxin | + | * '''Protein family:''' ferredoxin-NADP reductase type 2 family (according to Swiss-Prot) |
* '''Paralogous protein(s):''' [[YcgT]] | * '''Paralogous protein(s):''' [[YcgT]] | ||
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* '''[[Domains]]:''' | * '''[[Domains]]:''' | ||
| − | * '''Modification:''' active site Cys85 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species[http://www.ncbi.nlm.nih.gov/pubmed/22938038 PubMed] | + | * '''Modification:''' active site Cys85 is S-bacillithiolated by NaOCl stress in ''B. subtilis'' and other ''Bacillus'' species [http://www.ncbi.nlm.nih.gov/pubmed/22938038 PubMed] |
* '''[[Cofactors]]:''' FAD {{PubMed|20878669}} | * '''[[Cofactors]]:''' FAD {{PubMed|20878669}} | ||
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* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
** dimeric protein {{PubMed|20878669}} | ** dimeric protein {{PubMed|20878669}} | ||
| + | ** [[YumC]]-[[YkuN]] (electron transfer from NADPH via [[YumC]] to [[YkuN]]) {{PubMed|25194416}} | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
| Line 141: | Line 144: | ||
=References= | =References= | ||
| − | <pubmed>22938038, 20208166, 20878669, 19162251, 15252706 24529496 </pubmed> | + | <pubmed>22938038, 20208166, 20878669, 19162251, 15252706 24529496 25194416 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:32, 19 September 2014
- Description: ferredoxin/flavodoxin-NAD(P)+ oxidoreductase
| Gene name | yumC |
| Synonyms | |
| Essential | yes PubMed |
| Product | ferredoxin/flavodoxin reductase |
| Function | redox reactions that involve ferredoxin and flavodoxin |
| Gene expression levels in SubtiExpress: yumC | |
| Interactions involving this protein in SubtInteract: YumC | |
| MW, pI | 36 kDa, 5.573 |
| Gene length, protein length | 996 bp, 332 aa |
| Immediate neighbours | yumB, yuzG |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
electron transport/ other, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU32110
Phenotypes of a mutant
essential PubMed
Database entries
- BsubCyc: BSU32110
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2 reduced ferredoxin or flavodoxin + NADP+ + H+ = 2 oxidized ferredoxin or flavodoxin + NADPH (according to Swiss-Prot)
- Protein family: ferredoxin-NADP reductase type 2 family (according to Swiss-Prot)
- Paralogous protein(s): YcgT
Extended information on the protein
- Kinetic information:
- Modification: active site Cys85 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed
- Effectors of protein activity:
Database entries
- BsubCyc: BSU32110
- UniProt: O05268
- KEGG entry: [2]
- E.C. number: 1.18.1.2
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium): 5753 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 9972 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 5216 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 2849 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 3293 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
