mpfA

mpfA
168

primary magnesium efflux pump, mutation suppresses defects in 70 S ribosome formation

Locus
BSU_09550
Molecular weight
49.69 kDa
Isoelectric point
4.4
Protein length
444 aaSequenceBlast
Gene length
1335 bpSequenceBlast
Function
export of magnesium
Product
magnesium efflux pump
Essential
no
Synonyms
mpfA, yhdP
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1253 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,032,063 → 1,033,397
Phenotypes of a mutant
suppression of the growth defects of rplA and rplW mutants PubMed
partial suppression of sporulation frequency of a rplA mutant PubMed
suppresses the Mn2+ sensitivity of a mntR mntH double mutant PubMed
increased tolerance to Mn2+ and Co2+ PubMed
resistant to high Mg2+ concentrations PubMed
significantly delayed osmoadaptation to both high NaCl and high KCl PubMed
The protein
Protein family
UPF0053 family(according to UniProt)
Domains
CNNM transmembrane domain (aa 1-201) (according to UniProt)
DUF21 domain (aa 5-201)
2 CBS domains (aa 220-282, aa 284-344) (according to UniProt)
Structure
4HG0 (PDB) (CorC from E. coli, corresponds to the CBS domains and the C-terminal transporter-associated domain)
O07585 (AlphaFold)
Paralogous protein(s)
YqhB, YrkA, YugS, YhdT
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
yhdQ-mpfA
Description
PubMed
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yhdQ→mpfA

2025-04-03 20:35:16

ghost

85

ca09e60baea58b0b0fd5cf5f205da412921dc57f

EC6ED682C054AA1FE5B70E4649BB4B7C30DA995E

Biological materials
Mutant
HB24928 (ΔmpfA), available in John Helmann's and Jörg Stülke's labs
MGNA-A696 (yhdP::erm), available at the NBRP B. subtilis, Japan
BKE09550 (ΔmpfA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTAGAACCTTCACTCTA,  downstream forward: _UP4_TAATAAGAAAAGCACCTTGT
BKK09550 (ΔmpfA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTAGAACCTTCACTCTA,  downstream forward: _UP4_TAATAAGAAAAGCACCTTGT
Expression vectors
pGP2931 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
pGP3463 (214-349aa) (N-terminal 6xHis-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
pGP3468 (214-349aa) (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in Jörg Stülke's lab
References
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Page visits: 5496

Time of last update: 2025-04-10 01:29:38

Author of last update: Jstuelk