ssbB

ssbB
168

single-strand DNA-binding protein required to internalize and to recombine ssDNA with homologous resident duplex

Locus
BSU_36310
Molecular weight
12.35 kDa
Isoelectric point
6.88
Protein length
113 aaSequenceBlast
Gene length
342 bpSequenceBlast
Function
required for efficient genetic transformation
Product
single-strand DNA-binding protein
Essential
no
Synonyms
ssbB, ywpH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0629 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,740,206  3,740,547
The protein
Catalyzed reaction/ biological activity
binds and protects internalized foreign ssDNA PubMed
RecA-ATP in concert with DprA and SsbA catalyzes DNA strand exchange, with SsbB as an accessory factor PubMed
Domains
SSB domain (aa 1-104)  (according to UniProt)
Structure
3VDY (PDB) (in complex with ssDNA) PubMed
C0SPB6 (AlphaFold)
Modification
phosphorylated on Arg-55 PubMed
phosphorylated on Tyr by PtkA, dephosphorylated by PtpZ PubMed
Effectors of protein activity
phosphorylation strongly increases DNA-binding activity PubMed
Paralogous protein(s)
SsbA
Localization
localizes to cell poles in competent cells PubMed
localizes to the DNA entry pole during transformation PubMed
transiently localizes to the cell pole, and co-localizes with the DNA uptake machinery PubMed
Expression and Regulation
Operons
Genes
ssbB
Description
PubMed
Regulation
expressed at the onset of stationary phase PubMed
Regulatory mechanism
ComK: activation, PubMed, in comK regulon
Open in new tab

ssbB

2025-03-15 07:21:52

Jstuelk

95

fa3289f35dcfd53afbc7dd87da5ed09467f69ce4

4DC6BD6435E1CDE163200740137D096DDCC7CCCD

Genes
ywpG-ssbB
Description
PubMed
Open in new tab

ywpG→ssbB

2025-03-11 08:54:34

Jstuelk

91

548c9fdfd94a945c4d3b9c03dba2f900a800c3d6

BD6798D6ADE6A00697A155E4834F20B4FF866A7F

Biological materials
Mutant
GP3522 (ΔssbB::kan), available in Jörg Stülke's lab
MGNA-A591 (ywpH::erm), available at the NBRP B. subtilis, Japan
BKE36310 (ssbB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCGATTCCTCCTCTCCT,  downstream forward: _UP4_TGACACCCCTGCTCCTCCCG
BKK36310 (ssbB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCGATTCCTCCTCTCCT,  downstream forward: _UP4_TGACACCCCTGCTCCTCCCG
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
References
Reviews
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Original Publications
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1CDF658441061EA476E27C2E60DEE45C4F45AC15

Page visits: 4128

Time of last update: 2025-04-04 01:41:40

Author of last update: Jstuelk