DprA

• Description: conveys incoming ssDNA to RecA; facilitates the displacement of both SSBs (SsbB and SsbA), increases RecA nucleation onto SSB-coated ssDNA, and mediates DNA strand annealing, required to internalize and to recombine ssDNA with homologous resident duplex
  
  

Gene name	dprA
Synonyms	smf
Essential	no
Product	recombination mediator protein
Function	DNA repair/ recombination
Gene expression levels in SubtiExpress: dprA
Interactions involving this protein in SubtInteract: DprA
MW, pI	32 kDa, 9.755
Gene length, protein length	891 bp, 297 aa
Immediate neighbours	sucD, topA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, genetic competence

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU16110

Phenotypes of a mutant

• • strongly impaired genetic recombination PubMed

Database entries

• BsubCyc: BSU16110

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binds ssDNA, promotes displacement of SsbA and SsbB from ssDNA PubMed
  • provides RecA access to ssDNA during chromosomal transformation (together with RecO) PubMed

• Protein family: smf family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • forms dimers PubMed
  • DprA-RecA PubMed
  • DprA-DprA PubMed
  • DprA-ComFA PubMed
  • SsbB-DprA PubMed

• Localization:

Database entries

• BsubCyc: BSU16110

• Structure:

• UniProt: P39813

• KEGG entry: [3]

Expression and regulation

• Operon: dprA (according to DBTBS)

• Expression browser: dprA PubMed

• Sigma factor:

• Regulation:
  • expressed under conditions that trigger genetic competence (ComK) PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 57 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage