ugtP
168
UDP-glucose diacylglycerol glucosyltransferase, growth-rate dependent inhibitor of Cell division
Locus
BSU_21920
Molecular weight
43.40 kDa
Isoelectric point
8.4
Function
synthesis of glucolipids and anchoring of lipoteichoic acid, inhibition of FtsZ assembly
Product
UDP-glucose diacylglycerol glucosyltransferase
Essential
no
E.C.
2.4.1.315
Synonyms
ugtP, ypfP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0707 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,306,514 2,307,662
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth PubMed
increased levels of cell wall precursors PubMed
cells are bent and distended due to the lack of glucolipids PubMed
a ugtP lytE double mutant has a severe cell shape defect, thi can be suppressed by mutations resulting in reduced expression or activity of PbpA PubMed
the inactivation of ''ugtP suppresses the poor and filamentous growth of the whiA zapA'' double mutant PubMed
increased conjugation of ICEBs1 PubMed
The protein
Catalyzed reaction/ biological activity
1,2-diacyl-3-O-(β-D-glucopyranosyl)-sn-glycerol + UDP-α-D-glucose --> 1,2-diacyl-3-O-(β-D-Glc-(1→6)-β-D-Glc)-sn-glycerol + H+ + UDP (according to UniProt)
1,2-diacyl-3-O-(β-D-Glc-(1→6)-β-D-Glc)-sn-glycerol + UDP-α-D-glucose --> 1,2-diacyl-3-O-(β-D-Glc-(1→6)-β-D-Glc-(1→6)-β-D-Glc)-sn-glycerol + H+ + UDP (according to UniProt)
1,2-diacyl-sn-glycerol + UDP-α-D-glucose --> 1,2-diacyl-3-O-(β-D-glucopyranosyl)-sn-glycerol + H+ + UDP (according to UniProt)
the interaction with FtsZ results in inhibition of cell division and an increase of cell size PubMed
Protein family
Structure
4X1T (PDB) (galactolipid synthase from Arabidopsis thaliana, 25% identity) PubMed
Effectors of protein activity
Expression and Regulation
Biological materials
Mutant
MGNA-A886 (ypfP::erm), available at the NBRP B. subtilis, Japan
GP1369 (''ugtP::spc''), available in Jörg Stülke's lab
Expression vectors
pGP2571, for expression in B. subtilis (based on pBQ200, available in Jörg Stülke's lab
pGP2600, for expression/ purification from E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
References
Reviews
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Original Publications
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Time of last update: 2024-12-18 17:25:20
Author of last update: Jstuelk