ugtP

ugtP
168

UDP-glucose diacylglycerol glucosyltransferase, growth-rate dependent inhibitor of Cell division

Locus
BSU_21920
Molecular weight
43.40 kDa
Isoelectric point
8.4
Protein length
Gene length
Function
synthesis of glucolipids and anchoring of lipoteichoic acid, inhibition of FtsZ assembly
Product
UDP-glucose diacylglycerol glucosyltransferase
Essential
no
E.C.
2.4.1.315
Synonyms
ugtP, ypfP

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0707 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,306,514  2,307,662
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth PubMed
increased levels of cell wall precursors PubMed
cells are bent and distended due to the lack of glucolipids PubMed
a ugtP lytE double mutant has a severe cell shape defect, thi can be suppressed by mutations resulting in reduced expression or activity of PbpA PubMed
increased expression of the SigM, SigV, and SigX regulons PubMed
altered localization of MreB (irregular clusters instead of helical dots)  PubMed
the inactivation of ''ugtP suppresses the poor and filamentous growth of the whiA zapA'' double mutant PubMed
increased conjugation of ICEBs1 PubMed
The protein
Catalyzed reaction/ biological activity
1,2-diacyl-3-O-(β-D-glucopyranosyl)-sn-glycerol + UDP-α-D-glucose --> 1,2-diacyl-3-O-(β-D-Glc-(1→6)-β-D-Glc)-sn-glycerol + H+ + UDP (according to UniProt)
1,2-diacyl-3-O-(β-D-Glc-(1→6)-β-D-Glc)-sn-glycerol + UDP-α-D-glucose --> 1,2-diacyl-3-O-(β-D-Glc-(1→6)-β-D-Glc-(1→6)-β-D-Glc)-sn-glycerol + H+ + UDP (according to UniProt)
1,2-diacyl-sn-glycerol + UDP-α-D-glucose --> 1,2-diacyl-3-O-(β-D-glucopyranosyl)-sn-glycerol + H+ + UDP (according to UniProt)
the interaction with FtsZ results in inhibition of cell division and an increase of cell size PubMed
Protein family
glycosyltransferase 28 family (with YkoN and MurG, according to UniProt)
Structure
4X1T (PDB) (galactolipid synthase from Arabidopsis thaliana, 25% identity) PubMed
Effectors of protein activity
oligomerization of UgtP is inhibited by UDP-Glc and by interaction with FtsZ  PubMed
the amounts of UgtP are reduced about threefold during growth with poor carbon sources due to ClpP-dependent degradation (this requires most likely ClpX as the chaperone, but ClpC and ClpE are also effective) PubMed
membrane-bound protein, self-assembles into tightly wound spirals in vitro PubMed
under nutrient rich conditions (increased concentration of UDP-Glc): throughout the cell, concentrated at the cell poles and/or the cytokinetic ring, interaction with FtsZ PubMed
under nutrient poor conditions: forms punctate foci (oligomers), no interaction with FtsZ PubMed
Expression and Regulation
Operons
Genes
Description
Sigma factors
SigM: sigma factor, PubMed, in sigM regulon
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ugtP

2024-12-11 05:36:09

ghost

120

f785fc5b89e82870eac4bff6526ec42682b9329d

092EFC03C6BACF33E0E5FDD519063689156050F8

Genes
Description
Sigma factors
SigM: sigma factor, PubMed, in sigM regulon
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metAugtP

2024-12-11 14:48:44

ghost

112

45e856ff37b7ee445afb9c87f96abb81194264ae

4B45D0C21B6BA8DCA11EA8FC7ACD2A28343A2152

Additional information
the amounts of UgtP are reduced about threefold during growth with poor carbon sources due to ClpP-dependent degradation PubMed
Biological materials
Mutant
MGNA-A886 (ypfP::erm), available at the NBRP B. subtilis, Japan
GP1369 (''ugtP::spc''), available in Jörg Stülke's lab
BKE21920 (ugtP::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CAAGTAAATTCACCTCAATG,  downstream forward: _UP4_TAATGGCGTACTTGAGAGCA
BKK21920 (ugtP::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CAAGTAAATTCACCTCAATG,  downstream forward: _UP4_TAATGGCGTACTTGAGAGCA
Expression vectors
pGP2571, for expression in B. subtilis (based on pBQ200, available in Jörg Stülke's lab
pGP2600, for expression/ purification from E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
References
Reviews
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Original Publications
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7A606B8E952AE8CA4F9A62008BA4B156725BB5B5

Page visits: 8364

Time of last update: 2024-12-18 17:25:20

Author of last update: Jstuelk