Pta

• Description: phosphotransacetylase
  
  

Gene name	pta
Synonyms	ipa-88d, ywfJ
Essential	no
Product	phosphotransacetylase
Function	overflow metabolism
Gene expression levels in SubtiExpress: pta
Metabolic function and regulation of this protein in SubtiPathways: pta
MW, pI	34 kDa, 4.649
Gene length, protein length	969 bp, 323 aa
Immediate neighbours	cysL, ywfI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

• Locus tag: BSU37660

Database entries

• BsubCyc: BSU37660

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acetyl-CoA + phosphate = CoA + acetyl phosphate (according to Swiss-Prot)

• Protein family: phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on (Ser-123 OR Thr-128 OR Ser-129) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU37660

• Structure: 1XCO (complex with acetylphosphate), 1TD9

• UniProt: P39646

• KEGG entry: [3]

• E.C. number: 2.3.1.8

Expression and regulation

• Operon: pta PubMed

• Expression browser: pta PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expression activated by glucose (2.6 fold) (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 2825 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 7432 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 2878 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1417 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1754 PubMed

Biological materials

• Mutant: GP668 (aphA3), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• Antibody:

References

Reviews

Alan J Wolfe
The acetate switch.
Microbiol Mol Biol Rev: 2005, 69(1);12-50
[PubMed:15755952] [WorldCat.org] [DOI] (P p)

Original publications

Additional publications: PubMed

V K Morya, Varun Dewaker, Eun-Ki Kim
In silico study and validation of phosphotransacetylase (PTA) as a putative drug target for Staphylococcus aureus by homology-based modelling and virtual screening.
Appl Biochem Biotechnol: 2012, 168(7);1792-805
[PubMed:23054816] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Qian Steven Xu, Jarmila Jancarik, Yun Lou, Kate Kuznetsova, Alexander F Yakunin, Hisao Yokota, Paul Adams, Rosalind Kim, Sung-Hou Kim
Crystal structures of a phosphotransacetylase from Bacillus subtilis and its complex with acetyl phosphate.
J Struct Funct Genomics: 2005, 6(4);269-79
[PubMed:16283428] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

E Presecan-Siedel, A Galinier, R Longin, J Deutscher, A Danchin, P Glaser, I Martin-Verstraete
Catabolite regulation of the pta gene as part of carbon flow pathways in Bacillus subtilis.
J Bacteriol: 1999, 181(22);6889-97
[PubMed:10559153] [WorldCat.org] [DOI] (P p)

B S Shin, S K Choi, S H Park
Regulation of the Bacillus subtilis phosphotransacetylase gene.
J Biochem: 1999, 126(2);333-9
[PubMed:10423526] [WorldCat.org] [DOI] (P p)