lutA

lutA
168

lactate catabolic enzyme

Locus
BSU_34050
Molecular weight
26.24 kDa
Isoelectric point
6.25
Protein length
238 aaSequenceBlast
Gene length
717 bpSequenceBlast
Function
lactate utilization
Product
lactate oxidase
Essential
no
Synonyms
lutA, yvfV
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0247 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,494,985 → 3,495,701
Phenotypes of a mutant
no growth with lactate as the single carbon source PubMed
The protein
Catalyzed reaction/ biological activity
oxidation of lactate to pyruvate PubMed
Protein family
LutA/YkgE family (single member, according to UniProt)
Structure
5ODC (PDB) (from Methanothermococcus thermoautotrophicus, 24% identity) PubMed
O07020 (AlphaFold)
Expression and Regulation
Operons
Genes
lutA-lutB-lutC
Description
PubMed
Regulation
induction by lactate PubMed
Regulatory mechanism
SinR: repression, PubMed, in sinR regulon
LutR: repression, in lutR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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lutA→lutC

2025-03-15 03:34:41

ghost

92

7db2bc789fad5a99442f7b9ff99a43f2b42b4a12

59CEB598935063C72EA3D1BE77C54FD6CCE3442E

Other regulations
FsrA: translation repression, PubMed
FbpB: translation inhibition
Biological materials
Mutant
MGNA-A497 (yvfV::erm), available at the NBRP B. subtilis, Japan
BKE34050 (ΔlutA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGAACCCCTCTCTCA,  downstream forward: _UP4_TAAAACTGGATTCAGAGGGG
BKK34050 (ΔlutA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGAACCCCTCTCTCA,  downstream forward: _UP4_TAAAACTGGATTCAGAGGGG
LacZ fusion
GP1612 amyE::p(lutA-lacZ cat), constructed with pGP2149 based on pAC5, available in Jörg Stülke's lab
Labs working on this gene/protein
Richard Losick, Harvard Univ., Cambridge, USA homepage
References
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Page visits: 5425

Time of last update: 2025-04-02 04:05:29

Author of last update: Jstuelk