Difference between revisions of "YvcK"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34760 yvcK]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34760 yvcK]
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=YvcK YvcK]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 5.408   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 5.408   
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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[carbon core metabolism]]}}, {{SubtiWiki category|[[cell shape]]}}
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{{SubtiWiki category|[[carbon core metabolism]]}}, {{SubtiWiki category|[[cell shape]]}},
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{{SubtiWiki category|[[phosphoproteins]]}}
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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* '''Domains:'''  
 
* '''Domains:'''  
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** phosphorylated on Thr-304 by [[PrkC]], dephosphorylated by [[PrpC]] {{PubMed|25012659}}
  
 
* '''Modification:'''
 
* '''Modification:'''
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* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
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** [[YvcK]]-[[PrkC]] {{PubMed|25012659}}
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** [[YvcK]]-[[PrpC]] {{PubMed|25012659}}
  
* '''[[Localization]]:''' localized as a helical-like pattern {{PubMed|21320184}}
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* '''[[Localization]]:''' localized as a helical-like pattern {{PubMed|25012659,21320184}}
  
 
=== Database entries ===
 
=== Database entries ===
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==Original publications==
 
==Original publications==
<pubmed>9237995 16272399, 21320184 </pubmed>
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<pubmed>9237995 16272399, 21320184 25012659</pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 09:49, 12 July 2014

  • Description: essential for growth under gluconeogenetic conditions, required for correct localization of PBP1

Gene name yvcK
Synonyms
Essential no
Product unknown
Function localization of PBP1
Gene expression levels in SubtiExpress: yvcK
Interactions involving this protein in SubtInteract: YvcK
MW, pI 34 kDa, 5.408
Gene length, protein length 951 bp, 317 aa
Immediate neighbours yvcL, yvcJ
Sequences Protein DNA DNA_with_flanks
Genetic context
YvcK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YvcK expression.png















Categories containing this gene/protein

carbon core metabolism, cell shape, phosphoproteins

This gene is a member of the following regulons

Efp-dependent proteins

The gene

Basic information

  • Locus tag: BSU34760

Phenotypes of a mutant

  • unable to grow with gluconeogenic substrates as single carbon source PubMed
  • filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) PubMed, this is supressed by overexpression of MreB or by deletion of ponA (PBP1 ) PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: UPF0052 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: very weak stimuation of expression by citrate and succinate PubMed
  • Regulatory mechanism:
  • Additional information:
    • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 96 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 246 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 555 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 282 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 459 PubMed

Biological materials

  • Mutant:
  • Expression vector: pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
  • Antibody:

Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage

Anne Galinier, University of Marseille, France

Your additional remarks

References

Reviews

PubMed

Original publications