yyaN

yyaN
168

similar to transcriptional regulator (MerR family)

Locus
BSU_40800
Molecular weight
15.91 kDa
Isoelectric point
9.31
Protein length
138 aaSequenceBlast
Gene length
417 bpSequenceBlast
Function
unknown
Product
unknown
Essential
no
Synonyms
yyaN
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0789 (Galperin et al., 2021)

Gene
Coordinates
4,189,796  4,190,212
The protein
Protein family
MerR family
Domains
HTH merR-type domain (aa 3-72) (according to UniProt)
Structure
5E01 (PDB) (aa 2 ... 109, from Haemophilus influenzae, complex with DNA target, 39.5% identity) PubMed
P37510 (AlphaFold)
Expression and Regulation
Operons
Genes
yyaN-yyaM-yyaL
Description
PubMed
Open in new tab

yyaN→yyaL

2025-03-10 20:44:38

Jstuelk

90

dae36084e0a18eee42d8f69fcaaf519fcef43d33

C86B2672974B1551D3E5779BC0C0D78FB55E202D

Additional information
translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
Biological materials
Mutant
MGNA-B860 (yyaN::erm), available at the NBRP B. subtilis, Japan
BKE40800 (yyaN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATCCCTCTCATTTAAT,  downstream forward: _UP4_TTGAAAGAGGAAGTGGAAAA
BKK40800 (yyaN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATCCCTCTCATTTAAT,  downstream forward: _UP4_TTGAAAGAGGAAGTGGAAAA
Expression vectors
pGP3933 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
pGP3935 (C-terminal Strep-tag, purification from E. coli, in pGP574), available in Jörg Stülke's lab
References
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Page visits: 2282

Time of last update: 2025-03-14 21:26:54

Author of last update: Jstuelk