yyaN

yyaN
168

similar to transcriptional regulator (MerR family)

Locus
BSU_40800
Molecular weight
15.91 kDa
Isoelectric point
9.31
Protein length
Gene length
Function
unknown
Product
unknown
Essential
no
Synonyms
yyaN

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0789 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,189,796  4,190,212
The protein
Protein family
HTH merR-type domain (aa 3-72) (according to UniProt)
Structure
5E01 (PDB) (aa 2 ... 109, from Haemophilus influenzae, complex with DNA target, 39.5% identity) PubMed
Expression and Regulation
Operons
Description
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yyaNyyaL

2025-03-10 20:44:38

Jstuelk

90

dae36084e0a18eee42d8f69fcaaf519fcef43d33

C86B2672974B1551D3E5779BC0C0D78FB55E202D

Additional information
translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
Biological materials
Mutant
MGNA-B860 (yyaN::erm), available at the NBRP B. subtilis, Japan
BKE40800 (yyaN::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATCATCCCTCTCATTTAAT,  downstream forward: _UP4_TTGAAAGAGGAAGTGGAAAA
BKK40800 (yyaN::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATCATCCCTCTCATTTAAT,  downstream forward: _UP4_TTGAAAGAGGAAGTGGAAAA
Expression vectors
pGP3933 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
pGP3935 (C-terminal Strep-tag, purification from E. coli, in pGP574), available in Jörg Stülke's lab
References
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Time of last update: 2025-03-15 00:09:09

Author of last update: Jstuelk