Difference between revisions of "AdhR"

From SubtiWiki
Jump to: navigation, search
 
(14 intermediate revisions by 5 users not shown)
Line 1: Line 1:
−
* '''Description:''' transcriptional activator of ''[[adhA]]-[[yraA]]'' <br/><br/>
+
* '''Description:''' transcriptional activator ([[transcription factors of the MerR family|MerR family]]) of ''[[adhA]]-[[yraA]]'', responsive to formaldehyde and methylglyoxal <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
Line 10: Line 10:
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional activator ([[MerR family]])  
+
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional activator ([[transcription factors of the MerR family|MerR family]])
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of the protective response to formaldehyde
+
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of the protective response to formaldehyde and methylglyoxal
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27000 adhR]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 9.637   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 9.637   
Line 18: Line 20:
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 420 bp, 140 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 420 bp, 140 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yraD]]'', ''[[adhA]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yraD]]'', ''[[yrzP]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14642&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27000 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27000 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27000 DNA_with_flanks]
 
|-
 
|-
 
|-
 
|-
Line 26: Line 28:
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yraB_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yraB_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 +
|-
 +
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yraB_2755382_2755804_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:adhR_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27000]]
 
|-
 
|-
 
|}
 
|}
  
 
__TOC__
 
__TOC__
−
 
+
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 
<br/><br/>
 
<br/><br/>
  
Line 49: Line 55:
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27000&redirect=T BSU27000]
  
 
* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
Line 55: Line 62:
  
 
=== Additional information===
 
=== Additional information===
−
 
−
 
−
 
  
 
=The protein=
 
=The protein=
Line 65: Line 69:
 
* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
−
* '''Protein family:'''
+
* '''Protein family:''' [[transcription factors of the MerR family|MerR family]]
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
Line 75: Line 79:
 
* '''Domains:'''  
 
* '''Domains:'''  
  
−
* '''Modification:''' activity probably controlled via thiol-(S)-alkylation in Cys-52 {{PubMed|19170879}}
+
* '''Modification:''' activity probably redox-controlled via thiol-(S)-alkylation at Cys-52 by aldehydes {{PubMed|19170879}}
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
Line 83: Line 87:
 
* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
  
−
* '''Localization:''' spore wall (according to Swiss-Prot)
+
* '''[[Localization]]:''' cytoplasmic
−
 
 
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27000&redirect=T BSU27000]
  
 
* '''Structure:'''
 
* '''Structure:'''
Line 101: Line 105:
 
* '''Operon:'''  
 
* '''Operon:'''  
  
−
* '''[[Sigma factor]]:'''  
+
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yraB_2755382_2755804_-1 adhR] {{PubMed|22383849}}
  
−
* '''Regulation:'''  
+
* '''Sigma factor:'''
 +
 
 +
* '''Regulation:'''
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
−
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
Line 124: Line 130:
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 +
 +
[[Haike Antelmann]],University of Greifswald, Germany
  
 
=Your additional remarks=
 
=Your additional remarks=
Line 129: Line 137:
 
=References=
 
=References=
  
−
<pubmed>19170879,, </pubmed>
+
=Reviews=
 +
 
 +
<pubmed>21722790</pubmed>
 +
Antelmann H, Helmann JD.
 +
Thiol-based redox switches and gene regulation.
 +
Antioxid Redox Signal. 2011,14:1049-63.
 +
{{PubMed|20626317}}
 +
 
 +
=Original articles=
 +
 
 +
<pubmed>19170879</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Latest revision as of 13:14, 22 June 2014

  • Description: transcriptional activator (MerR family) of adhA-yraA, responsive to formaldehyde and methylglyoxal

Gene name adhR
Synonyms yraB
Essential no
Product transcriptional activator (MerR family)
Function regulation of the protective response to formaldehyde and methylglyoxal
Gene expression levels in SubtiExpress: adhR
MW, pI 16 kDa, 9.637
Gene length, protein length 420 bp, 140 aa
Immediate neighbours yraD, yrzP
Sequences Protein DNA DNA_with_flanks
Genetic context
YraB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AdhR expression.png















Categories containing this gene/protein

transcription factors and their control, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU27000

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: activity probably redox-controlled via thiol-(S)-alkylation at Cys-52 by aldehydes PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Haike Antelmann,University of Greifswald, Germany

Your additional remarks

References

Reviews

Alastair G McEwan, Karrera Y Djoko, Nathan H Chen, Rafael L M Couñago, Stephen P Kidd, Adam J Potter, Michael P Jennings
Novel bacterial MerR-like regulators their role in the response to carbonyl and nitrosative stress.
Adv Microb Physiol: 2011, 58;1-22
[PubMed:21722790] [WorldCat.org] [DOI] (I p) 

Antelmann H, Helmann JD.
Thiol-based redox switches and gene regulation.
Antioxid Redox Signal. 2011,14:1049-63.
PubMed

Original articles

Thi Thu Huyen Nguyen, Warawan Eiamphungporn, Ulrike Mäder, Manuel Liebeke, Michael Lalk, Michael Hecker, John D Helmann, Haike Antelmann
Genome-wide responses to carbonyl electrophiles in Bacillus subtilis: control of the thiol-dependent formaldehyde dehydrogenase AdhA and cysteine proteinase YraA by the MerR-family regulator YraB (AdhR).
Mol Microbiol: 2009, 71(4);876-94
[PubMed:19170879] [WorldCat.org] [DOI] (I p)

Retrieved from "http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=AdhR&oldid=205890"