ansR

ansR
168

transcriptional repressor (Xre family) of the ansA-ansB operon

Locus
BSU_23590
Molecular weight
13.09 kDa
Isoelectric point
4.81
Protein length
116 aaSequenceBlast
Gene length
351 bpSequenceBlast
Function
negative regulation of the ansA-ansB operon
Product
transcriptional regulator (Xre family)
Essential
no
Synonyms
ansR
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG1396 (Galperin et al., 2021)

This gene is a member of the following regulons

AnsR regulon

Gene
Coordinates
2,456,990  2,457,340
Phenotypes of a mutant
the ansR mutation allows glutamate utilization of a rocG gudB mutant PubMed
The protein
Protein family
Xre family
Domains
HTH cro/C1-type domain (aa 6-60) (according to UniProt)
Structure
Q07683 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
ansR
Description
PubMed
Regulation
induced in the presence of asparagine (AnsR) PubMed
Regulatory mechanism
AnsR: negative autoregulation, undefined, in ansR regulon
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ansR

2025-03-09 09:49:26

ghost

84

6b2490649352aba044e93aaa313de73854805f48

2EA9DD8814DE48AF76A41D5CD8E2735D2B188FA6

Biological materials
Mutant
GP4166 (ansR::cat), available in  Jörg Stülke's lab
GP1152 (ΔansR::tet) available in Jörg Stülke's lab PubMed
BKE23590 (ansR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAACTTCCGCTCCTTTT,  downstream forward: _UP4_GAGGAACTGAGTTAATCTTT
BKK23590 (ansR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAACTTCCGCTCCTTTT,  downstream forward: _UP4_GAGGAACTGAGTTAATCTTT
Expression vectors
expression of native ansR in B. subtilis: pGP873 (in pBQ200), available in Jörg Stülke's lab
References
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146947C974D62BCB282BA3F5B924B35695D75886

Page visits: 3814

Time of last update: 2025-04-06 06:05:58

Author of last update: Jstuelk