yesT

yesT
168

rhamnogalacturonan acetylesterase

Locus
BSU_07020
Molecular weight
25.80 kDa
Isoelectric point
5.51
Protein length
232 aaSequenceBlast
Gene length
699 bpSequenceBlast
Function
pectin utilization
Product
rhamnogalacturonan esterase
Essential
no
Synonyms
yesT
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2755 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
768,137  768,835
The protein
Protein family
'GDSL' lipolytic enzyme family (according to UniProt)
Structure
2O14 (PDB) (YxiM, 29% identity)
O31523 (AlphaFold)
Paralogous protein(s)
YxiM, YesY
Expression and Regulation
Operons
Genes
rhiL-rhiF-rhiG-rhiN-rhgR-yesT-yesU-yesV-yesW-yesX-yesY-yesZ
Description
PubMed
Regulation
induced by pectin (RhgR, RhgL) PubMed
repressed by glucose (CcpA) PubMed
Regulatory mechanism
RhgR: activation, PubMed, in rhgR regulon
SigA: Sigma factor, PubMed, in sigA regulon
RhgL: activation, PubMed, in rhgL regulon
CcpA: repression, PubMed, in ccpA regulon
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rhiL→yesZ

2025-03-16 17:43:25

Jstuelk

117

D891C8F38E150B653BA655BA6352352D86D18BAE

A10D4124F5AF6831808648D33C98AF061D3703AA

Biological materials
Mutant
MGNA-B451 (yesT::erm), available at the NBRP B. subtilis, Japan
BKE07020 (yesT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCTTCATCACATGTTCTC,  downstream forward: _UP4_CTTGTGAGCCGGGAGGGAAA
BKK07020 (yesT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCTTCATCACATGTTCTC,  downstream forward: _UP4_CTTGTGAGCCGGGAGGGAAA
References
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585CA3154C0405697C4707E5B1D9359963999C54

Page visits: 2172

Time of last update: 2025-04-10 20:19:39

Author of last update: Melvin.boenninger