rhiN

rhiN
168

galacturonyl hydrolase, catalyses intracellular degradation of disaccharides generated by YesX

Locus
BSU_07000
Molecular weight
38.52 kDa
Isoelectric point
4.76
Protein length
344 aaSequenceBlast
Gene length
1035 bpSequenceBlast
Function
intracellular degradation of disaccharides generated by YesX
Product
galacturonyl hydrolase, catalyses intracellular degradation of disaccharides generated by YesX
Essential
no
Synonyms
rhiN, yesR
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4225 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
764,781  765,815
The protein
Catalyzed reaction/ biological activity
2-O-(4-deoxy-β-L-threo-hex-4-enopyranuronosyl)-α-L-rhamnopyranose + H2O --> 5-dehydro-4-deoxy-D-glucuronate + L-rhamnopyranose (according to UniProt)
Protein family
glycosyl hydrolase 105 family (with YteR, according to UniProt)
Structure
3PMM (PDB) (from Klebsiella pneumoniae, 30% identity)
O31521 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
rhiL-rhiF-rhiG-rhiN-rhgR-yesT-yesU-yesV-yesW-yesX-yesY-yesZ
Description
PubMed
Regulation
induced by pectin (RhgR, RhgL) PubMed
repressed by glucose (CcpA) PubMed
Regulatory mechanism
RhgR: activation, PubMed, in rhgR regulon
SigA: Sigma factor, PubMed, in sigA regulon
RhgL: activation, PubMed, in rhgL regulon
CcpA: repression, PubMed, in ccpA regulon
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rhiL→yesZ

2025-03-16 17:43:25

Jstuelk

117

D891C8F38E150B653BA655BA6352352D86D18BAE

A10D4124F5AF6831808648D33C98AF061D3703AA

Biological materials
Mutant
MGNA-B260 (yesR::erm), available at the NBRP B. subtilis, Japan
BKE07000 (rhiN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTGTGCCATCTGCAACACTC,  downstream forward: _UP4_TAAAGATAGCGGCGGGGGGA
BKK07000 (rhiN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTGTGCCATCTGCAACACTC,  downstream forward: _UP4_TAAAGATAGCGGCGGGGGGA
References
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8EED04FCE6E2900B729178209FB281F5463B2540

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Time of last update: 2025-04-06 01:59:52

Author of last update: Melvin.boenninger