rhiG

rhiG
168

ABC transporter (membrane protein) for rhamnose oligosaccharides

Locus
BSU_06990
Molecular weight
33.32 kDa
Isoelectric point
9.52
Protein length
296 aaSequenceBlast
Gene length
891 bpSequenceBlast
Function
uptake of rhamnose oligosaccharides
Product
rhamnose oligosaccharide ABC transporter
Essential
no
Synonyms
rhiG, yesQ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0395 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
763,875  764,765
The protein
Protein family
Binding-protein-dependent transport system permease family (according to UniProt)
MalFG subfamily (according to UniProt)
Domains
ABC transmembrane type-1 domain (aa 91-282) (according to UniProt)
Structure
4TQU (PDB) (from Sphingomonas sp., 25% identity) PubMed
O31520 (AlphaFold)
Localization
cell membrane PubMed
Expression and Regulation
Operons
Genes
rhiL-rhiF-rhiG-rhiN-rhgR-yesT-yesU-yesV-yesW-yesX-yesY-yesZ
Description
PubMed
Regulation
induced by pectin (RhgR, RhgL) PubMed
repressed by glucose (CcpA) PubMed
Regulatory mechanism
RhgR: activation, PubMed, in rhgR regulon
SigA: Sigma factor, PubMed, in sigA regulon
RhgL: activation, PubMed, in rhgL regulon
CcpA: repression, PubMed, in ccpA regulon
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rhiL→yesZ

2025-03-16 17:43:25

Jstuelk

117

D891C8F38E150B653BA655BA6352352D86D18BAE

A10D4124F5AF6831808648D33C98AF061D3703AA

Biological materials
Mutant
MGNA-A949 (yesQ::erm), available at the NBRP B. subtilis, Japan
BKE06990 (rhiG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGGCTGGTTGACCGGCTCCA,  downstream forward: _UP4_TTAAAATAAAAAGGAGTGTT
BKK06990 (rhiG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGGCTGGTTGACCGGCTCCA,  downstream forward: _UP4_TTAAAATAAAAAGGAGTGTT
References
Mahendran A, Orlando BJGenome wide structural prediction of ABC transporter systems in Bacillus subtilis.Frontiers in microbiology. 2024; 15:1469915. PMID: 39397791
O'Reilly FJ, Graziadei A, Forbrig C, Bremenkamp R, Charles K, Lenz S, Elfmann C, Fischer L, Stülke J, Rappsilber JProtein complexes in cells by AI-assisted structural proteomics.Molecular systems biology. 2023 Feb 23; :e11544. PMID: 36815589
Maruyama Y, Itoh T, Kaneko A, Nishitani Y, Mikami B, Hashimoto W, Murata K Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate. Structure (London, England : 1993). 2015 Sep 01; 23(9):1643-54. doi:10.1016/j.str.2015.06.021. pii:S0969-2126(15)00274-9. PMID:26235029
Rodionova IA, Li X, Thiel V, Stolyar S, Stanton K, Fredrickson JK, Bryant DA, Osterman AL, Best AA, Rodionov DA Comparative genomics and functional analysis of rhamnose catabolic pathways and regulons in bacteria. Frontiers in microbiology. 2013; 4:407. doi:10.3389/fmicb.2013.00407. PMID:24391637
Ochiai A, Itoh T, Kawamata A, Hashimoto W, Murata K Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization. Applied and environmental microbiology. 2007 Jun; 73(12):3803-13. . PMID:17449691
Quentin Y, Fichant G, Denizot F Inventory, assembly and analysis of Bacillus subtilis ABC transport systems. Journal of molecular biology. 1999 Apr 02; 287(3):467-84. . PMID:10092453

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Time of last update: 2025-04-04 08:30:48

Author of last update: Jstuelk