SigB

• Description: RNA polymerase sigma factor SigB
  
  

Gene name	sigB
Synonyms	rpoF
Essential	no
Product	RNA polymerase sigma factor SigB
Function	general stress response
Gene expression levels in SubtiExpress: sigB
Interactions involving this protein in SubtInteract: SigB
Metabolic function and regulation of this protein in SubtiPathways: sigB
MW, pI	29 kDa, 5.418
Gene length, protein length	792 bp, 264 aa
Immediate neighbours	rsbW, rsbX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, sigma factors and their control, general stress proteins (controlled by SigB)

This gene is a member of the following regulons

CcpA regulon, SigB regulon

The gene

Basic information

• Locus tag: BSU04730

Database entries

• BsubCyc: BSU04730

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• mutations in mtrB, sigB, rpoB, and rpoC allow B. subtilis to grow with 4-fluorotryptophan rather than with tryptophan as a canonical amino acid of the genetic code PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: SigB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • SigB-RsbW PubMed
  • SigB-RpoB-RpoC

• Localization:

Database entries

• BsubCyc: BSU04730

• Structure:

• UniProt: P06574

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • rsbR-rsbS-rsbT-rsbU-rsbV-rsbW-sigB-rsbX PubMed
  • rsbV-rsbW-sigB-rsbX PubMed

• Expression browser: sigB PubMed

• Sigma factor:
  • rsbR: SigA PubMed
  • rsbV: SigB PubMed

• Regulation:
  • rsbV: induced by stress (SigB) PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 52 PubMed

Biological materials

• Mutant: QB5344 (cat), available in the Stülke lab
  • 1A675 ( sigB::cat), PubMed, available at BGSC
  • 1A777 ( sigB::cat), PubMed, available at BGSC
  • 1A780 ( sigB::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Bill Haldenwang, San Antonio, USA
• Chet Price, Davis, USA homepage

References

Reviews

Jon Marles-Wright, Richard J Lewis
The stressosome: molecular architecture of a signalling hub.
Biochem Soc Trans: 2010, 38(4);928-33
[PubMed:20658979] [WorldCat.org] [DOI] (I p)

Peter Zuber
Management of oxidative stress in Bacillus.
Annu Rev Microbiol: 2009, 63;575-97
[PubMed:19575568] [WorldCat.org] [DOI] (I p)

Michael Hecker, Jan Pané-Farré, Uwe Völker
SigB-dependent general stress response in Bacillus subtilis and related gram-positive bacteria.
Annu Rev Microbiol: 2007, 61;215-36
[PubMed:18035607] [WorldCat.org] [DOI] (P p)

M Hecker, U Völker
General stress response of Bacillus subtilis and other bacteria.
Adv Microb Physiol: 2001, 44;35-91
[PubMed:11407115] [WorldCat.org] [DOI] (P p)

M Hecker, U Völker
Non-specific, general and multiple stress resistance of growth-restricted Bacillus subtilis cells by the expression of the sigmaB regulon.
Mol Microbiol: 1998, 29(5);1129-36
[PubMed:9767581] [WorldCat.org] [DOI] (P p)

Control of SigB activity by protein-protein interactions

Oleg A Igoshin, Margaret S Brody, Chester W Price, Michael A Savageau
Distinctive topologies of partner-switching signaling networks correlate with their physiological roles.
J Mol Biol: 2007, 369(5);1333-52
[PubMed:17498739] [WorldCat.org] [DOI] (P p)

Tae-Jong Kim, Tatiana A Gaidenko, Chester W Price
In vivo phosphorylation of partner switching regulators correlates with stress transmission in the environmental signaling pathway of Bacillus subtilis.
J Bacteriol: 2004, 186(18);6124-32
[PubMed:15342582] [WorldCat.org] [DOI] (P p)

Tae-Jong Kim, Tatiana A Gaidenko, Chester W Price
A multicomponent protein complex mediates environmental stress signaling in Bacillus subtilis.
J Mol Biol: 2004, 341(1);135-50
[PubMed:15312768] [WorldCat.org] [DOI] (P p)

Olivier Delumeau, Richard J Lewis, Michael D Yudkin
Protein-protein interactions that regulate the energy stress activation of sigma(B) in Bacillus subtilis.
J Bacteriol: 2002, 184(20);5583-9
[PubMed:12270815] [WorldCat.org] [DOI] (P p)

M S Brody, K Vijay, C W Price
Catalytic function of an alpha/beta hydrolase is required for energy stress activation of the sigma(B) transcription factor in Bacillus subtilis.
J Bacteriol: 2001, 183(21);6422-8
[PubMed:11591687] [WorldCat.org] [DOI] (P p)

C Eymann, M Hecker
Induction of sigma(B)-dependent general stress genes by amino acid starvation in a spo0H mutant of Bacillus subtilis.
FEMS Microbiol Lett: 2001, 199(2);221-7
[PubMed:11377871] [WorldCat.org] [DOI] (P p)

A Dufour, U Voelker, A Voelker, W G Haldenwang
Relative levels and fractionation properties of Bacillus subtilis σ(B) and its regulators during balanced growth and stress.
J Bacteriol: 1996, 178(13);3701-9 sigma
[PubMed:8682769] [WorldCat.org] [DOI] (P p)

U Voelker, A Voelker, B Maul, M Hecker, A Dufour, W G Haldenwang
Separate mechanisms activate sigma B of Bacillus subtilis in response to environmental and metabolic stresses.
J Bacteriol: 1995, 177(13);3771-80
[PubMed:7601843] [WorldCat.org] [DOI] (P p)

A A Wise, C W Price
Four additional genes in the sigB operon of Bacillus subtilis that control activity of the general stress factor sigma B in response to environmental signals.
J Bacteriol: 1995, 177(1);123-33
[PubMed:8002610] [WorldCat.org] [DOI] (P p)

A K Benson, W G Haldenwang
Bacillus subtilis sigma B is regulated by a binding protein (RsbW) that blocks its association with core RNA polymerase.
Proc Natl Acad Sci U S A: 1993, 90(6);2330-4
[PubMed:8460143] [WorldCat.org] [DOI] (P p)

Identification of the SigB regulon

Priyanka Nannapaneni, Falk Hertwig, Maren Depke, Michael Hecker, Ulrike Mäder, Uwe Völker, Leif Steil, Sacha A F T van Hijum
Defining the structure of the general stress regulon of Bacillus subtilis using targeted microarray analysis and random forest classification.
Microbiology (Reading): 2012, 158(Pt 3);696-707
[PubMed:22174379] [WorldCat.org] [DOI] (I p)

J D Helmann, M F Wu, P A Kobel, F J Gamo, M Wilson, M M Morshedi, M Navre, C Paddon
Global transcriptional response of Bacillus subtilis to heat shock.
J Bacteriol: 2001, 183(24);7318-28
[PubMed:11717291] [WorldCat.org] [DOI] (P p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

C W Price, P Fawcett, H Cérémonie, N Su, C K Murphy, P Youngman
Genome-wide analysis of the general stress response in Bacillus subtilis.
Mol Microbiol: 2001, 41(4);757-74
[PubMed:11532142] [WorldCat.org] [DOI] (P p)

A Petersohn, J Bernhardt, U Gerth, D Höper, T Koburger, U Völker, M Hecker
Identification of sigma(B)-dependent genes in Bacillus subtilis using a promoter consensus-directed search and oligonucleotide hybridization.
J Bacteriol: 1999, 181(18);5718-24
[PubMed:10482513] [WorldCat.org] [DOI] (P p)

Anja Petersohn, Haike Antelmann, Ulf Gerth, Michael Hecker
Identification and transcriptional analysis of new members of the sigmaB regulon in Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 4);869-880
[PubMed:10220166] [WorldCat.org] [DOI] (P p)