ykuO

ykuO
168

unknown

Locus
BSU_14160
Molecular weight
33.46 kDa
Isoelectric point
8.13
Protein length
297 aaSequenceBlast
Gene length
894 bpSequenceBlast
Function
unknown
Product
unknown
Essential
no
Synonyms
ykuO
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms

This gene is a member of the following regulons

SigA regulon, ResD regulon, NsrR regulon, Fur regulon, SigI regulon, Kre regulon

Gene
Coordinates
1,487,504 → 1,488,397
The protein
Structure
O34879 (AlphaFold)
Expression and Regulation
Operons
Genes
ykuN-ykuO-ykuP
Description
PubMed
Regulation
expressed under anaerobic conditions (ResD) PubMed
induced by iron starvation (second wave) (Fur) PubMed
Regulatory mechanism
ResD: activation, PubMed, in resD regulon
Kre: repression, in kre regulon
NsrR: repression, PubMed, in nsrR regulon
Fur: repression, PubMed, in fur regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigI: sigma factor, PubMed, in sigI regulon
Additional information
the ''YkuN-YkuO-YkuP operon is strongly unregulated in a kre'' mutant PubMed
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ykuN→ykuP

2025-04-03 19:50:10

Jstuelk

97

57db5b9bbc0032750db2eb9bfdbed1110c747af0

E599E3DBF7B4049028BE0EE09C6A470E8B1A923E

Biological materials
Mutant
GP4731 ΔykuO::aphA3, available in Jörg Stülke's lab
MGNA-B338 (ykuO::erm), available at the NBRP B. subtilis, Japan
BKE14160 (ΔykuO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGATGCCGTAACAGCGGTTG,  downstream forward: _UP4_ATTCATTAGAGGAGGAACAA
BKK14160 (ΔykuO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGATGCCGTAACAGCGGTTG,  downstream forward: _UP4_ATTCATTAGAGGAGGAACAA
References
Ramaniuk O, Převorovský M, Pospíšil J, Vítovská D, Kofroňová O, Benada O, Schwarz M, Šanderová H, Hnilicová J, Krásný L σ from : Impact on Gene Expression and Characterization of σ-dependent Transcription that Requires New Types of Promoters with Extended -35 and -10 Elements. Journal of bacteriology. 2018 Jun 18; . pii:JB.00251-18. doi:10.1128/JB.00251-18. PMID:29914988
Pi H, Helmann JD Sequential induction of Fur-regulated genes in response to iron limitation in Bacillus subtilis. Proceedings of the National Academy of Sciences of the United States of America. 2017 Nov 13; . pii:201713008. doi:10.1073/pnas.1713008114. PMID:29133393
Gamba P, Jonker MJ, Hamoen LW A Novel Feedback Loop That Controls Bimodal Expression of Genetic Competence. PLoS genetics. 2015 Jun; 11(6):e1005047. doi:10.1371/journal.pgen.1005047. PMID:26110430
Henares B, Kommineni S, Chumsakul O, Ogasawara N, Ishikawa S, Nakano MM The ResD response regulator, through functional interaction with NsrR and fur, plays three distinct roles in Bacillus subtilis transcriptional control. Journal of bacteriology. 2014 Jan; 196(2):493-503. doi:10.1128/JB.01166-13. PMID:24214949
Baichoo N, Wang T, Ye R, Helmann JD Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon. Molecular microbiology. 2002 Sep; 45(6):1613-29. . PMID:12354229
Mäder U, Homuth G, Scharf C, Büttner K, Bode R, Hecker M Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability. Journal of bacteriology. 2002 Aug; 184(15):4288-95. . PMID:12107147

CEC1B5DF1E548597FE333895ECA476AB9DFFFDD7

Page visits: 5089

Time of last update: 2025-04-06 03:43:06

Author of last update: Jstuelk