NrgB

• Description: nitrogen-regulated PII-like protein
  
  

Gene name	nrgB
Synonyms	glnK
Essential	no
Product	nitrogen-regulated PII-like protein
Function	regulation of ammonium uptake
Gene expression levels in SubtiExpress: nrgB
Interactions involving this protein in SubtInteract: NrgB
Metabolic function and regulation of this protein in SubtiPathways: nrgB
MW, pI	12 kDa, 8.825
Gene length, protein length	348 bp, 116 aa
Immediate neighbours	nrgA, bcrC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, glutamate metabolism, transcription factors and their control, membrane proteins

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

• Locus tag: BSU36520

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: P(II) protein family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • NrgA-NrgB PubMed
  • TnrA-NrgB PubMed
  • GlnA-NrgB PubMed

• Localization: cell membrane, via NrgA PubMed

Database entries

• Structure: 2NUU (the complex with NrgA, from E. coli) PubMed

• UniProt: Q07428

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nrgA-nrgB PubMed

• Expression browser: nrgB PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by ammonium limitation
  • induced by ammonium limitation (TnrA) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: GP259 (cat), GP255 (nrgA-nrgB, cat), both available in Jörg Stülke's lab PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Jörg Stülke lab

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

References

Reviews

Cheng-Cai Zhang, Cong-Zhao Zhou
ATPase as a switch in P(II) signal transduction.
Proc Natl Acad Sci U S A: 2013, 110(32);12863-4
[PubMed:23847201] [WorldCat.org] [DOI] (I p)

Karl Forchhammer
P(II) signal transducers: novel functional and structural insights.
Trends Microbiol: 2008, 16(2);65-72
[PubMed:18182294] [WorldCat.org] [DOI] (P p)

T Arcondéguy, R Jack, M Merrick
P(II) signal transduction proteins, pivotal players in microbial nitrogen control.
Microbiol Mol Biol Rev: 2001, 65(1);80-105
[PubMed:11238986] [WorldCat.org] [DOI] (P p)

G Thomas, G Coutts, M Merrick
The glnKamtB operon. A conserved gene pair in prokaryotes.
Trends Genet: 2000, 16(1);11-4
[PubMed:10637624] [WorldCat.org] [DOI] (P p)