lipA
168

lipoyl synthase, trigger enzyme

Locus

BSU_32330

Molecular weight

33.77 kDa

Isoelectric point

8.25

Protein length

298 aaSequenceBlast

Gene length

897 bpSequenceBlast

Function

synthesis of lipoic acid

Product

lipoyl synthase,trigger enzyme

Essential

E.C.

2.8.1.8

Synonyms

lipA, yutB

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0320 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

3,320,324 3,321,220

Phenotypes of a mutant

reduced transformation efficiency PubMed

strong inhibition of growth on minimal media PubMed

The protein

Catalyzed reaction/ biological activity

[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S]²⁺ cluster] + 4 H⁺ + N⁶-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine --> (R)-N⁶-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe³⁺ + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin] (according to UniProt)

required for ''comEA'' transcription PubMed

Protein family

Radical SAM superfamily (according to UniProt)

Cofactors

Fe-S cluster PubMed

Structure

4U0O (PDB) (from Thermosynechococcus elongatus, 48% identity) PubMed

O32129 (AlphaFold)

Expression and Regulation

Operons

Genes

lipA