YwrD

• Description: unknown, similar to gamma-glutamyltransferase (Ggt)
  
  

Gene name	ywrD
Synonyms
Essential	no
Product	unknown
Function	unknown
Gene expression levels in SubtiExpress: ywrD
MW, pI	57 kDa, 5.451
Gene length, protein length	1575 bp, 525 aa
Immediate neighbours	ywrE, chrS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteins of unknown function

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

• Locus tag: BSU36100

Database entries

• BsubCyc: BSU36100

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl amino acid (according to Swiss-Prot), however, all evidence suggests this is a misannotation PubMed

• Protein family: gamma-glutamyltransferase family (according to Swiss-Prot), however, all evidence suggests this is a misannotation PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU36100

• Structure:

• UniProt: O05218

• KEGG entry: [3]

• E.C. number: 2.3.2.2

Additional information

The gene is annotated in KEGG as an ortholog of gamma-glutamyltranspeptidase EC 2.3.2.2, This enzyme is necessary to utilize glutathione (GSH) as the sulfur source. No EC annotation for this gene is available in Swiss-Prot/MetaCyc. It has been shown by Minami et al. (PubMed) that ywrD mutant grows well on minimal media supplied with GSH as the sole sulfur source. In addition, His-tag purified ywrD cannot hydrolyze GSH. PubMed

Expression and regulation

• Operon: ywrD PubMed

• Expression browser: ywrD PubMed

• Sigma factor:

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hiromichi Minami, Hideyuki Suzuki, Hidehiko Kumagai
Gamma-glutamyltranspeptidase, but not YwrD, is important in utilization of extracellular glutathione as a sulfur source in Bacillus subtilis.
J Bacteriol: 2004, 186(4);1213-4
[PubMed:14762019] [WorldCat.org] [DOI] (I p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)