ComP

• Description: two-component sensor kinase, detects extracellular ComX
  
  

Gene name	comP
Synonyms
Essential	no
Product	two-component sensor kinase
Function	regulation of genetic competence and quorum sensing
Gene expression levels in SubtiExpress: comP
Interactions involving this protein in SubtInteract: ComP
MW, pI	89 kDa, 7.997
Gene length, protein length	2307 bp, 769 aa
Immediate neighbours	comA, comX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, protein modification, transcription factors and their control, quorum sensing, membrane proteins, phosphoproteins

The gene

Basic information

• Locus tag: BSU31690

Phenotypes of a mutant

• the mutation suppresses the mucoid phenotype of motA or motB mutants due to loss of DegU phosphorylation and concomitant reduced expression of the capB-capC-capA-capE operon PubMed

Database entries

• BsubCyc: BSU31690

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of ComA

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: eight transmembrane segments

• Modification: autophosphorylation on a His residue

• Cofactors:

• Effectors of protein activity: ComX (extracellular)

• Interactions:
  • ComP-ComA

• Localization: cell membrane PubMed

Database entries

• BsubCyc: BSU31690

• Structure:

• UniProt: Q99027

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: comP PubMed

• Expression browser: comP PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Leendert W Hamoen, Gerard Venema, Oscar P Kuipers
Controlling competence in Bacillus subtilis: shared use of regulators.
Microbiology (Reading): 2003, 149(Pt 1);9-17
[PubMed:12576575] [WorldCat.org] [DOI] (P p)

Original Publications

Iztok Dogsa, Kumari Sonal Choudhary, Ziva Marsetic, Sanjarbek Hudaiberdiev, Roberto Vera, Sándor Pongor, Ines Mandic-Mulec
ComQXPA quorum sensing systems may not be unique to Bacillus subtilis: a census in prokaryotic genomes.
PLoS One: 2014, 9(5);e96122
[PubMed:24788106] [WorldCat.org] [DOI] (I e)

Anna Oslizlo, Polonca Stefanic, Iztok Dogsa, Ines Mandic-Mulec
Private link between signal and response in Bacillus subtilis quorum sensing.
Proc Natl Acad Sci U S A: 2014, 111(4);1586-91
[PubMed:24425772] [WorldCat.org] [DOI] (I p)

Jia Mun Chan, Sarah B Guttenplan, Daniel B Kearns
Defects in the flagellar motor increase synthesis of poly-γ-glutamate in Bacillus subtilis.
J Bacteriol: 2014, 196(4);740-53
[PubMed:24296669] [WorldCat.org] [DOI] (I p)

Xidan Deng, Yunxia Tian, Qiuhong Niu, Xiao'e Xu, Hui Shi, Hanbo Zhang, Lianming Liang, Keqin Zhang, Xiaowei Huang
The ComP-ComA quorum system is essential for "Trojan horse" like pathogenesis in Bacillus nematocida.
PLoS One: 2013, 8(10);e76920
[PubMed:24130811] [WorldCat.org] [DOI] (I e)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Mireille Ansaldi, Darja Marolt, Tina Stebe, Ines Mandic-Mulec, David Dubnau
Specific activation of the Bacillus quorum-sensing systems by isoprenylated pheromone variants.
Mol Microbiol: 2002, 44(6);1561-73
[PubMed:12067344] [WorldCat.org] [DOI] (P p)

P Tortosa, L Logsdon, B Kraigher, Y Itoh, I Mandic-Mulec, D Dubnau
Specificity and genetic polymorphism of the Bacillus competence quorum-sensing system.
J Bacteriol: 2001, 183(2);451-60
[PubMed:11133937] [WorldCat.org] [DOI] (P p)

F Piazza, P Tortosa, D Dubnau
Mutational analysis and membrane topology of ComP, a quorum-sensing histidine kinase of Bacillus subtilis controlling competence development.
J Bacteriol: 1999, 181(15);4540-8
[PubMed:10419951] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)

Bauke Oudega, Gregory Koningstein, Luísa Rodrigues, Maria de Sales Ramon, Helmut Hilbert, Andreas Düsterhöft, Thomas M Pohl, Thomas Weitzenegger
Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai).
Microbiology (Reading): 1997, 143 ( Pt 8);2769-2774
[PubMed:9274030] [WorldCat.org] [DOI] (P p)

M Perego, J A Hoch
Cell-cell communication regulates the effects of protein aspartate phosphatases on the phosphorelay controlling development in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1996, 93(4);1549-53
[PubMed:8643670] [WorldCat.org] [DOI] (P p)

Y Weinrauch, R Penchev, E Dubnau, I Smith, D Dubnau
A Bacillus subtilis regulatory gene product for genetic competence and sporulation resembles sensor protein members of the bacterial two-component signal-transduction systems.
Genes Dev: 1990, 4(5);860-72
[PubMed:2116363] [WorldCat.org] [DOI] (P p)