AbnA

• Description: endo-1,5-alpha-L-arabinosidase
  
  

Gene name	abnA
Synonyms
Essential	no
Product	endo-1,5-alpha-L-arabinosidase
Function	arabinan degradation
Gene expression levels in SubtiExpress: abnA
MW, pI	34 kDa, 8.618
Gene length, protein length	939 bp, 313 aa
Immediate neighbours	araA, ysdC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, sporulation/ other

This gene is a member of the following regulons

AraR regulon, CcpA regulon

The gene

Basic information

• Locus tag: BSU28810

Database entries

• BsubCyc: BSU28810

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endohydrolysis of (1->5)-alpha-arabinofuranosidic linkages in (1->5)-arabinans (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 43 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• BsubCyc: BSU28810

• Structure: 1UV4

• UniProt: P94522

• KEGG entry: [3]

• E.C. number: 3.2.1.99

Expression and regulation

• Operon: abnA PubMed

• Expression browser: abnA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the absence of arabinose (AraR) PubMed
  • subject to carbon catabolite repression (CcpA) PubMed
• Regulatory mechanism:
  • AraR: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: