ywjH

ywjH
168

transaldolase

Locus
BSU_37110
Molecular weight
22.77 kDa
Isoelectric point
5.88
Protein length
212 aaSequenceBlast
Gene length
639 bpSequenceBlast
Function
pentose phosphate pathway
Product
transaldolase
Essential
no
E.C.
2.2.1.2
Synonyms
ywjH, tal
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0176 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,807,754 3,808,392
The protein
Catalyzed reaction/ biological activity
D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate --> -D-fructose 6-phosphate + D-erythrose 4-phosphate (according to UniProt)
Protein family
transaldolase family (single member, according to UniProt)
Structure
3R8R (PDB) PubMed
P19669 (AlphaFold)
Modification
phosphorylation on Ser-39 PubMed, in vitro phosphorylated by PrkC PubMed
Localization
cytoplasm (according to Swiss-Prot)
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
spo0F-fbaA-ywjH-murAB-glpX-rho-rpmE
Description
PubMed
Expression
readthrough-transcription from the SigH-dependent promoter after the initiation of sporulation in the mother cell PubMed
Open in new tab

spo0F→rpmE

2025-03-16 05:53:33

Jstuelk

27

C63B272CC9442316212569FF9E0451D7AD583B70

C10FBD1184EE96831E587248498E99020C63607C

Genes
fbaA-ywjH
Description
PubMed
Regulation
constitutively expressed PubMed
Open in new tab

fbaA→ywjH

2025-04-01 20:23:49

ghost

116

3de4c158d0f59e42a135e3344384ecf580467739

F733D4A7CF3474C9704F8FE6039F28B670F0B03B

Biological materials
Mutant
MGNA-B264 (ywjH::erm), available at the NBRP B. subtilis, Japan
BKE37110 (ywjH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTCAAAAAGCCTCCCT, downstream forward: _UP4_TAATGAAAGGGGCGGCAAAC
BKK37110 (ywjH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTCAAAAAGCCTCCCT, downstream forward: _UP4_TAATGAAAGGGGCGGCAAAC
Expression vectors
for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP819, available in Jrg Stlke's lab, PubMed
pGP1790 (expression of ''ywjH in B. subtilis'', in pGP1389), available in Jrg Stlke's lab
GP1409 (ywjH-Strep (spc)) & GP1411 (ywjH-Strep (cat)), purification from B. subtilis, for SPINE, available in Jrg Stlke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jrg Stlke's lab
FLAG-tag construct
GP1407 (spc, based on pGP1331), available in Jrg Stlke's lab
References
Reviews
Loading
Original Publications
Loading

1419F2F99D924973D75EE6FAA41F9A27EC5283E1

Page visits: 4428

Time of last update: 2025-04-03 14:55:52

Author of last update: Jstuelk