bacF

bacF
168

aminotransferase involved in bacilysin synthesis

Locus
BSU_37690
Molecular weight
44.54 kDa
Isoelectric point
5.35
Protein length
399 aaSequenceBlast
Gene length
1200 bpSequenceBlast
Function
biosynthesis of the antibiotic bacilysin
Product
pyridoxal 5-phosphate-dependent stereospecific aminotransferase
Essential
no
E.C.
2.6.1.1
Synonyms
bacF, ywfG, ipa-85d
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0436 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,868,287  3,869,486
The protein
Catalyzed reaction/ biological activity
YwfG catalyzes transamination of tetrahydro-4-hydroxyphenylpyruvate (with L-Phe as amino donor), to form tetrahydrotyrosine PubMed
Protein family
class-I pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)
Cofactors
PLP
Structure
6L1L (PDB) (apo-protein) PubMed
6L1N (PDB) (substrate-bound) PubMed
6L1O (PDB) (product-bound) PubMed
2O1B (PDB) (from Staphylococcus aureus, 40% identity)
P39643 (AlphaFold)
Paralogous protein(s)
MtnE
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
bacA-bacB-bacC-bacD-bacE-bacF
Description
PubMed
Regulation
heterogeneous expression, percentage of expressing cells increases during colonization of plant roots PubMed
Regulatory mechanism
CodY: repression, PubMed, in codY regulon
AbrB: repression, PubMed, in abrB regulon
ScoC: repression, PubMed, in scoC regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

bacA→bacF

2025-03-26 18:53:52

Jstuelk

112

7D644717829C1A41F65E52A4312081846FB55951

33F4BF1A14F6AC22A6ACC7091140A7F012E3837C

Biological materials
Mutant
MGNA-A514 (ywfG::erm), available at the NBRP B. subtilis, Japan
BKE37690 (bacF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GACATCGGACGGTGTTATTT,  downstream forward: _UP4_TCCCGCTAAAAAGCGGGATG
BKK37690 (bacF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GACATCGGACGGTGTTATTT,  downstream forward: _UP4_TCCCGCTAAAAAGCGGGATG
References
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99434727013FCCD6E570AE550560A8546F7F2A41

Page visits: 4990

Time of last update: 2025-04-04 06:02:51

Author of last update: Jstuelk