ilvK

ilvK
168

branched-chain amino acid aminotransferase

Locus
BSU_38550
Molecular weight
40.16 kDa
Isoelectric point
4.95
Protein length
363 aaSequenceBlast
Gene length
1092 bpSequenceBlast
Function
biosynthesis of branched-chain amino acids
Product
branched-chain amino acid aminotransferase
Essential
no
E.C.
2.6.1.42
Synonyms
ywaA, ipa-0r
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0115 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,957,391 3,958,482
The protein
Catalyzed reaction/ biological activity
2-oxoglutarate + L-leucine --> 4-methyl-2-oxopentanoate + L-glutamate (according to UniProt)
2-oxoglutarate + L-isoleucine --> (S)-3-methyl-2-oxopentanoate + L-glutamate (according to UniProt)
2-oxoglutarate + L-valine --> 3-methyl-2-oxobutanoate + L-glutamate (according to UniProt)
Protein family
Class-IV pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)
Cofactors
PLP
Structure
3HT5 (PDB) (from Mycobacterium tuberculosis, 42% identity, 58% similarity) PubMed
P39576 (AlphaFold)
Modification
S-cysteinylation after diamide stress (C104) PubMed
Paralogous protein(s)
IlvE
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
ilvK
Description
PubMed
Regulation
repressed during growth in the presence of branched chain amino acids (CodY) PubMed
Regulatory mechanism
CodY: repression, PubMed, in codY regulon
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ilvK

2025-03-24 05:55:29

ghost

120

651105c8ef41c65a8034fda4ffb818741e12316f

AEEBD14281489BB37128A0CEDE8C71348D61CE7B

Biological materials
Mutant
MGNA-B757 (ywaA::erm), available at the NBRP B. subtilis, Japan
a ywaA::spc mutant and a ''bcd ilvE ilvK'' triple mutant are available in Linc Sonenshein's lab
BKE38550 (ilvK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGTCAATCTCCCTGCTG, downstream forward: _UP4_TAAGAAAAAAGCCGGCCCAT
BKK38550 (ilvK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGTCAATCTCCCTGCTG, downstream forward: _UP4_TAAGAAAAAAGCCGGCCCAT
References
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8163996FCB9D02F771E7A04A91D5720027260F12

Page visits: 6162

Time of last update: 2025-04-04 01:35:19

Author of last update: Robert.warneke