CysJ

• Description: sulfite reductase (NADPH2)
  
  

Gene name	cysJ
Synonyms	yvgQ
Essential	no
Product	sulfite reductase (NADPH2)
Function	sulfite reduction
Gene expression levels in SubtiExpress: cysJ
Metabolic function and regulation of this protein in SubtiPathways: cysJ
MW, pI	64 kDa, 5.49
Gene length, protein length	1713 bp, 571 aa
Immediate neighbours	nhaK, cysI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sulfur metabolism, most abundant proteins

This gene is a member of the following regulons

CysL regulon

The gene

Basic information

• Locus tag: BSU33430

Phenotypes of a mutant

• unable to grow with sulfate or sulfite as the only sulfur source PubMed

Database entries

• BsubCyc: BSU33440

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors: contains an iron-sulfur cluster

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU33440

• Structure:

• UniProt: O32214

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cysI-cysJ PubMed

• Expression browser: cysJ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by sulfite, sulfate, and thiosulfate (CysL) PubMed
  • repressed by casamino acids PubMed

• Regulatory mechanism:
  • CysL: transcription activation PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 2899 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1652 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1863 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 824 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 899 PubMed

Biological materials

• Mutant:
  • 1A802 ( cysJ::kan), PubMed, available at BGSC
  • 1A934 ( cysJ::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)

Isabelle Guillouard, Sandrine Auger, Marie-Françoise Hullo, Farid Chetouani, Antoine Danchin, Isabelle Martin-Verstraete
Identification of Bacillus subtilis CysL, a regulator of the cysJI operon, which encodes sulfite reductase.
J Bacteriol: 2002, 184(17);4681-9
[PubMed:12169591] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

J R van der Ploeg, M Barone, T Leisinger
Functional analysis of the Bacillus subtilis cysK and cysJI genes.
FEMS Microbiol Lett: 2001, 201(1);29-35
[PubMed:11445163] [WorldCat.org] [DOI] (P p)