cysI

cysI
168

sulfite reductase (NADPH2) (beta subunit)

Locus
BSU_33430
Molecular weight
64.63 kDa
Isoelectric point
5.49
Protein length
571 aaSequenceBlast
Gene length
1716 bpSequenceBlast
Function
sulfite reduction
Product
sulfite reductase (NADPH2)
Essential
no
Synonyms
cysI, yvgR
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0155 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,430,598  3,432,313
Phenotypes of a mutant
unable to grow with sulfate or sulfite as the only sulfur source PubMed
The protein
Catalyzed reaction/ biological activity
3 H2O + hydrogen sulfide + 3 NADP+ --> 4 H+ + 3 NADPH + sulfite (according to UniProt)
Protein family
nitrite and sulfite reductase 4Fe-4S domain family (with NasD, according to UniProt)
Cofactors
Fe-S cluster PubMed
heme PubMed
Structure
6C3M (PDB) (from E. coli, 52% identity) PubMed
O32213 (AlphaFold)
Expression and Regulation
Operons
Genes
cysI-cysJ
Description
PubMed
Regulation
induced by sulfite, sulfate, and thiosulfate (CysL) PubMed
Regulatory mechanism
CysL: activation, PubMed, in cysL regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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cysI→cysJ

2025-04-03 23:03:20

ghost

101

c76dbdd9ed7d8724551be3211cb722c3be05ea7a

CF5DADBE8969C7BB4BA71235FA6595D0555E76EC

Biological materials
Mutant
MGNA-B602 (yvgR::erm), available at the NBRP B. subtilis, Japan
1A802 ( cysI::kan), PubMed, available at BGSC
1A934 ( cysI::kan), PubMed, available at BGSC
BKE33430 (''cysI::erm'', available in the BGSC, in Fabian Commichau's, and in Jörg Stülke's lab) PubMed
BKE33430 (cysI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTGAAAAACTCCTTTC,  downstream forward: _UP4_TGAGAAAGAAAGACGAGAGA
BKK33430 (cysI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTGAAAAACTCCTTTC,  downstream forward: _UP4_TGAGAAAGAAAGACGAGAGA
References
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A4C9E4D1163B703A917BFCF97A5F575B19EDDD8D

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Time of last update: 2025-04-03 04:49:47

Author of last update: Jstuelk