nrnA

nrnA
168

oligoribonuclease (nanoRNase), 3,5-bisphosphate nucleotidase, degradation of pGpG

Locus
BSU_29250
Molecular weight
34.93 kDa
Isoelectric point
4.67
Protein length
313 aaSequenceBlast
Gene length
942 bpSequenceBlast
Function
processing of short RNA substrates
Product
oligoribonuclease (nanoRNase), 3,5-bisphosphate nucleotidase
Essential
no
E.C.
3.1.3.7
Synonyms
nrnA, ytqI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0618 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,995,908  2,996,849
Phenotypes of a mutant
The mutant requires cysteine PubMed
strongly reduced genetic competence, competence can be restored by overexpression of comK-comS PubMed
inactivation of nrnA reduces sporulation efficiency to 5% that of wild type cells; delayed entry into sporulation, reduced SigG activity, and production of small forespores PubMed
The protein
Catalyzed reaction/ biological activity
oligoribonuclease (nanoRNAse), 3',5'-bisphosphate nucleotidase PubMed
hydrolyzes oligo(deoxy)ribonucleotides in a 5' 3' direction PubMed
hydrolyzes nano-RNAs in a 3' 5' direction PubMed
degradation of pGpG derived from c-di-GMP PubMed
adenosine 3',5'-bisphosphate + H2O --> AMP + phosphate (according to UniProt)
Protein family
NrnA oligoribonuclease family (single member, according to UniProt)
Domains
DHH-DHHA1 domain PubMed
Cofactors
Mn2+ PubMed
Structure
5J21 (PDB) PubMed
O34600 (AlphaFold)
Localization
Cytoplasm (Homogeneous) PubMed
Expression and Regulation
Operons
Genes
nrnA
Description
PubMed
Open in new tab

nrnA

2025-03-15 20:09:35

ghost

84

39a6f64addb8b628f471fb6331b60617e7e93698

561C540607A61C60D840036C75C966A226C078BB

Biological materials
Mutant
MGNA-A151 (ytqI::erm), available at the NBRP B. subtilis, Japan
GP2155 (ΔnrnA::aphA3), available in Jörg Stülke's lab PubMed
BKE29250 (nrnA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGATACTCCTTTATTG,  downstream forward: _UP4_GAGTAGAGGGGAGACCCTCT
BKK29250 (nrnA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGATACTCCTTTATTG,  downstream forward: _UP4_GAGTAGAGGGGAGACCCTCT
References
Reviews
Loading
Original Publications
Loading

4C630B61249F8F2369E9BFE8A49885D3225DE7B6

Page visits: 4248

Time of last update: 2025-04-10 19:31:59

Author of last update: Jstuelk