Difference between revisions of "BshB1"
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* '''Additional information:''' | * '''Additional information:''' | ||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 343 {{PubMed|21395229}} | ||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 419 {{PubMed|21395229}} | ||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 618 {{PubMed|21395229}} | ||
=Biological materials = | =Biological materials = | ||
− | |||
* '''Mutant:''' ''bshB1'' null mutant and ''[[bshB1]]'' / ''[[bshB2]]'' double null mutant available in [[John Helmann]] lab | * '''Mutant:''' ''bshB1'' null mutant and ''[[bshB1]]'' / ''[[bshB2]]'' double null mutant available in [[John Helmann]] lab | ||
** GP87 (''ypjG''::''pX2''(''cat'')), available in [[Stülke]] lab | ** GP87 (''ypjG''::''pX2''(''cat'')), available in [[Stülke]] lab |
Revision as of 14:20, 17 April 2014
- Description: N-acetylglucosamin-malate deacetylase, involved in bacillithiol synthesis (major enzyme)
Gene name | bshB1 |
Synonyms | jojG, ypjG |
Essential | no |
Product | N-acetylglucosamin-malate deacetylase |
Function | biosynthesis of bacillithiol |
Gene expression levels in SubtiExpress: bshB1 | |
Metabolic function and regulation of this protein in SubtiPathways: bshB1 | |
MW, pI | 24 kDa, 6.343 |
Gene length, protein length | 672 bp, 224 aa |
Immediate neighbours | bshA, mgsA |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
miscellaneous metabolic pathways, resistance against oxidative and electrophile stress
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU22470
Phenotypes of a mutant
Database entries
- BsubCyc: BSU22470
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: GlcNAc-Mal -> GlcN-Mal + Ac
- Protein family:
- Paralogous protein(s): BshB2
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- BsubCyc: BSU22470
- Structure:
- UniProt: P42981
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 343 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 419 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 618 PubMed
Biological materials
- Mutant: bshB1 null mutant and bshB1 / bshB2 double null mutant available in John Helmann lab
- GP87 (ypjG::pX2(cat)), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References