yneT

yneT
168

similar to predicted CoA-binding protein with NAD(P)-binding Rossmann-fold domain

Locus
BSU_18080
Molecular weight
14.85 kDa
Isoelectric point
5.28
Protein length
135 aaSequenceBlast
Gene length
408 bpSequenceBlast
Function
unknown
Product
unknown
Essential
no
Synonyms
yneT
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1832 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,932,671 → 1,933,078
The protein
Structure
2DUW (PDB) (from Klebsiella pneumoniae, 40% identity)
Q45065 (AlphaFold)
Modification
CoA is attached to Cys-126 in spores PubMed
Expression and Regulation
Operons
Genes
yneT
Description
PubMed
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yneT

2025-03-23 00:13:36

Jstuelk

56

d0ddf07a9db47180b8bba4a0760e38df797504c2

F2BA6BA23C5CF350ACEBE5AF89AE0FBD39193AE2

Additional information
the mRNA is cleaved by RNase III PubMed
Biological materials
Mutant
MGNA-B393 (yneT::erm), available at the NBRP B. subtilis, Japan
BKE18080 (ΔyneT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTGTGTCACTCCTTTT,  downstream forward: _UP4_TAGGTGCAGAACTGCAAAAA
BKK18080 (ΔyneT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTGTGTCACTCCTTTT,  downstream forward: _UP4_TAGGTGCAGAACTGCAAAAA
References
Zhyvoloup A, Yu BYK, Baković J, Davis-Lunn M, Tossounian MA, Thomas N, Tsuchiya Y, Peak-Chew SY, Wigneshweraraj S, Filonenko V, Skehel M, Setlow P, Gout IAnalysis of disulphide bond linkage between CoA and protein cysteine thiols during sporulation and in spores of Bacillus species.FEMS microbiology letters. 2020 Dec 22; 367(23). PMID: 33206970
DiChiara JM, Liu B, Figaro S, Condon C, Bechhofer DH Mapping of internal monophosphate 5' ends of Bacillus subtilis messenger RNAs and ribosomal RNAs in wild-type and ribonuclease-mutant strains. Nucleic acids research. 2016 Apr 20; 44(7):3373-89. doi:10.1093/nar/gkw073. PMID:26883633

EB52009B0D8EE1B3B90842C8DE6E46C008C801CE

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Time of last update: 2025-04-06 10:58:45

Author of last update: Jstuelk