fakA

fakA
168

fatty acid kinase

Locus
BSU_15840
Molecular weight
59.32 kDa
Isoelectric point
4.28
Protein length
553 aaSequenceBlast
Gene length
1662 bpSequenceBlast
Function
phosphorylation of fatty acids
Product
fatty acid kinase
Essential
no
Synonyms
fakA, yloV
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG1461 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,656,442  1,658,103
The protein
Catalyzed reaction/ biological activity
phosphorylation of fatty acids (bound to DegV or YitS) PubMed
Domains
kinase domain (DhaL domain) (aa 1-202) PubMed
middle (dimerization) domain (aa 239-321) PubMed
C-terminal domain (aa 333-548) PubMed
Cofactors
Zn (2+), binds to middle domain PubMed
Structure
8VIP (PDB) (from S. aureus, N-terminal domain) (67% identity) PubMed
7RM7 (PDB) (from S. aureus, N-terminal domain) (67% identity)
6W6B (PDB) (from S. aureus, C-terminal domain) (48% identity)
O34751 (AlphaFold)
Modification
phosphorylated on Arg-255 PubMed
Additional information
subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and Regulation
Operons
Genes
rfaB-fakA
Description
PubMed
Open in new tab

rfaB→fakA

2025-02-15 15:41:43

Bzhu

85

59df383c358e285f8f394d9d07619671e9b5d639

60743BCE1ECE1AB944532A871551DEAAB9A8E78C

Biological materials
Mutant
MGNA-B137 (fakA::erm), available at the NBRP B. subtilis, Japan
GP1466 (fakA::cat), available in Jörg Stülke's lab
GP1467 (rfaB - fakA::cat), available in Jörg Stülke's lab PubMed
BKE15840 (fakA::erm trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GATAGACAATCCTACTCCTC,  downstream forward: _UP4_TAGAAGGGCAATTTGCCCTT
BKK15840 (fakA::kan trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GATAGACAATCCTACTCCTC,  downstream forward: _UP4_TAGAAGGGCAATTTGCCCTT
Expression vectors
pGP3623 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in Jörg Stülke's lab
pGP3624 expression of Strep-fakA by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
pGP3631 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
pGP3643 (C-terminal Strep-tag, purification from E. coli, in pGP574), available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
FLAG-tag construct
GP3780 fakA-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab
References
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5DE73E8005AFFD30EB84696B2C2474899C6D1982

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Time of last update: 2025-04-06 10:58:45

Author of last update: Jstuelk