yfmT

yfmT
168

vanillin dehydrogenase

Locus
BSU_07350
Molecular weight
53.15 kDa
Isoelectric point
5.08
Protein length
485 aaSequenceBlast
Gene length
1458 bpSequenceBlast
Function
unknown
Product
vanillin dehydrogenase
Essential
no
Synonyms
yfmT
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1012 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
807,091 → 808,548
The protein
Catalyzed reaction/ biological activity
oxidation of vanillin to vanillic acid PubMed
benzaldehyde + H2O + NAD+ --> benzoate + 2 H+ + NADH (according to UniProt)
4-hydroxy-3-methoxybenzaldehyde + H2O + NAD+ --> 4-hydroxy-3-methoxybenzoate + 2 H+ + NADH (according to UniProt)
Protein family
aldehyde dehydrogenase family (according to UniProt)
Structure
4DNG (PDB) (from Bacillus Subtilis, 47% identity)
3RHH (PDB) (from from Bacillus halodurans C-125 complexed with NADP, 32% identity, 67% similarity)
O06478 (AlphaFold)
Paralogous protein(s)
AldX, AldY, GbsA, YcbD, DhaS, IolA, YwdH, GabD, PutC, RocA
Expression and Regulation
Operons
Genes
yfmT-yfmS
Description
PubMed
Sigma factors
SigD: sigma factor, PubMed, in sigD regulon
Additional information
in minimal medium, YfmS is present with 4,200 +/- 800 molecules per cell PubMed
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yfmT→yfmS

2025-03-10 07:18:37

ghost

68

1ecc984aac2b0819e1aa3401111de8ee84edde7b

F5A7965A9921A9579F965D5324320AD715850A5B

Biological materials
Mutant
MGNA-C232 (yfmT::erm), available at the NBRP B. subtilis, Japan
BKE07350 (ΔyfmT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTCAGCATCTCCTTTA,  downstream forward: _UP4_TTCCCTTATTAATGAAAAGG
BKK07350 (ΔyfmT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTCAGCATCTCCTTTA,  downstream forward: _UP4_TTCCCTTATTAATGAAAAGG
Labs working on this gene/protein
Josef Altenbuchner
References
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EF0AAAF5BAE8E1F54FCA296643F7B9E3CE257B1D

Page visits: 2930

Time of last update: 2025-04-10 17:51:23

Author of last update: Melvin.boenninger