purR

purR
168

transcription repressor of the pur operon

Locus
BSU_00470
Molecular weight
31.08 kDa
Isoelectric point
5.77
Protein length
285 aaSequenceBlast
Gene length
858 bpSequenceBlast
Function
regulation of purine biosynthesis
Product
transcription repressor of the pur operon
Essential
no
Synonyms
purR, yabI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0503 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
54,441  55,298
The protein
Protein family
Purine/pyrimidine phosphoribosyltransferase family (according to UniProt)
Domains
N-terminal winged helix-turn-helix domain PubMed
C-terminal effector binding domain PubMed
Structure
1O57 (PDB) (the apo-protein) PubMed
7RMW (PDB) (complex with ppGpp) PubMed
P37551 (AlphaFold)
Effectors of protein activity
PRPP acts as inducer, binding results in release of PurR from the operator PubMed
(p)ppGpp acts as an anti-inducer, prevents binding of the inducer PRPP PubMed
Kinetic information
KD for ppGpp: 4.4 µM PubMed
Expression and Regulation
Operons
Genes
ispE-purR-yabJ
Description
PubMed
Regulation
negative autoregulation PubMed
Regulatory mechanism
PurR: repression, PubMed, in purR regulon
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ispE→yabJ

2025-03-31 01:13:41

Jstuelk

115

620be59868c14a2ea15f75e6fdf1022628ca65bb

B42ADE39EB92F53783BD0BDEF129E37859FEBF47

Biological materials
Mutant
BKE00470 (purR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAACTCACCCCCAAAAT,  downstream forward: _UP4_TTAAAGAATGGAGAGACAGA
BKK00470 (purR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAACTCACCCCCAAAAT,  downstream forward: _UP4_TTAAAGAATGGAGAGACAGA
References
Reviews
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Original Publications
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Time of last update: 2025-04-05 23:10:46

Author of last update: Jstuelk