purQ

purQ
168

phosphoribosylformylglycinamidine synthase

Locus
BSU_06470
Molecular weight
24.64 kDa
Isoelectric point
4.63
Protein length
227 aaSequenceBlast
Gene length
684 bpSequenceBlast
Function
purine biosynthesis
Product
phosphoribosylformylglycinamidine synthase
Essential
no
E.C.
6.3.5.3
Synonyms
purQ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0047 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
702,570 → 703,253
The protein
Catalyzed reaction/ biological activity
ATP + H2O + L-glutamine + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide --> 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + ADP + H+ + L-glutamate + phosphate(according to UniProt)
H2O + L-glutamine --> L-glutamate + NH4+ (according to UniProt)
Domains
Glutamine amidotransferase type-1 domain (aa 3-225) (according to UniProt)
Structure
3D54 (PDB) (from Thermotoga maritima, 51% identity) PubMed
P12041 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Additional information
subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and Regulation
Operons
Genes
purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD
Description
PubMed
Regulation
expression activated by glucose (4.4 fold) PubMed
Regulatory mechanism
G-box: RNA switch, (riboswitch) PubMed, in G-box
PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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purE→purD

2025-04-06 02:18:42

Jstuelk

231

ace69dc80f4f087486c218727fa370a7a9630d21

EDD8EC37B0E5B38941D5827C265AB2F23CF23EA7

Biological materials
Mutant
BKE06470 (ΔpurQ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGGTAACACAATCACCGCAA,  downstream forward: _UP4_ATCGTGAAAAATTGGAGGGA
BKK06470 (ΔpurQ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGGTAACACAATCACCGCAA,  downstream forward: _UP4_ATCGTGAAAAATTGGAGGGA
References
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DEE856E83E790EB57F103F7A9156408AF090995D

Page visits: 5648

Time of last update: 2025-04-05 05:58:42

Author of last update: Melvin.boenninger