Difference between revisions of "Hag"

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** number of protein molecules per cell (minimal medium with glucose and ammonium): 150241 {{PubMed|24696501}}
 
** number of protein molecules per cell (minimal medium with glucose and ammonium): 150241 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 280953 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 280953 {{PubMed|24696501}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 9552 {{PubMed|21395229}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 11147 {{PubMed|21395229}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 24114 {{PubMed|21395229}}
  
 
=Biological materials =
 
=Biological materials =
 
 
* '''Mutant:''' GP901 (aphA3), GP902 (tet) {{PubMed|21856853}}, both available in [[Jörg Stülke]]'s lab  
 
* '''Mutant:''' GP901 (aphA3), GP902 (tet) {{PubMed|21856853}}, both available in [[Jörg Stülke]]'s lab  
 
** 1A915 ( ''hag''::''cat''), {{PubMed|19202088}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A915&Search=1A915 BGSC]
 
** 1A915 ( ''hag''::''cat''), {{PubMed|19202088}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A915&Search=1A915 BGSC]

Revision as of 14:08, 17 April 2014

  • Description: flagellin protein, about 20,000 subunits make up one flagellum

Gene name hag
Synonyms
Essential no
Product flagellin protein
Function motility and chemotaxis
Gene expression levels in SubtiExpress: hag
Interactions involving this protein in SubtInteract: Hag
Metabolic function and regulation of this protein in SubtiPathways:
Hag
MW, pI 32 kDa, 4.782
Gene length, protein length 912 bp, 304 aa
Immediate neighbours yvyC, csrA
Sequences Protein DNA DNA_with_flanks
Genetic context
Hag context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Hag expression.png















Categories containing this gene/protein

motility and chemotaxis, most abundant proteins

This gene is a member of the following regulons

CodY regulon, CsrA regulon, ScoC regulon, SigD regulon

The gene

Basic information

  • Locus tag: BSU35360

Phenotypes of a mutant

No swarming motility on B medium. PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: bacterial flagellin family (according to Swiss-Prot)
  • Paralogous protein(s): YvzB (C-terminal domain of Hag)

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 3A5X (from Salmonella typhimurium, 42% identity)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:
    • the hag gene is strongly overexpressed in a ymdB mutant (loss of bistable gene expression) PubMed
    • belongs to the 100 most abundant proteins PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 150241 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 280953 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 9552 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 11147 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 24114 PubMed

Biological materials

  • Expression vector:
  • GFP fusion: BP494 (bglS:: (hag-promoter-cfp-aphA3)), BP496 (amyE:: (hag-promoter-iyfp-cat)), available in Jörg Stülke's lab
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Tony Romeo, Christopher A Vakulskas, Paul Babitzke
Post-transcriptional regulation on a global scale: form and function of Csr/Rsm systems.
Environ Microbiol: 2013, 15(2);313-24
[PubMed:22672726] [WorldCat.org] [DOI] (I p)


Original Publications