cysH

cysH
168

phosphoadenosine phosphosulfate sulfotransferase

Locus
BSU_15570
Molecular weight
26.83 kDa
Isoelectric point
5.46
Protein length
233 aaSequenceBlast
Gene length
702 bpSequenceBlast
Function
sulfate reduction
Product
phosphoadenosine phosphosulfate sulfotransferase
Essential
no
E.C.
1.8.4.8
Synonyms
cysH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0175 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,630,382  1,631,083
The protein
Catalyzed reaction/ biological activity
[thioredoxin]-disulfide + adenosine 3',5'-bisphosphate + 2 H+ + sulfite --> 3'-phosphoadenylyl sulfate + [thioredoxin]-dithiol (according to UniProt)
Protein family
PAPS reductase family (with YitB, according to UniProt)
Structure
2GOY (PDB) (from Pseudomonas aeruginosa, 35% identity) PubMed
P94498 (AlphaFold)
Paralogous protein(s)
YitB
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
cysH-cysP-sat-cysC-ylnD-sirB-ylnF
Description
PubMed
Regulation
induced by methionine starvation (S-box) PubMed
the S-box RNA is degraded by RNase Y PubMed
Regulatory mechanism
CymR: transcription repression, [pubmed|], in cymR regulon
S-box: RNA switch, , the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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cysH→ylnF

2025-03-26 23:14:36

Jstuelk

129

ee26ed9bec04d4ffdc2deea174a197c7290dfe30

AC610BA23AE4EE98F22EC54E46509E3060BAE285

Biological materials
Mutant
BKE15570 (cysH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCTTCTTTCCTCCTTT,  downstream forward: _UP4_CTGCATGAATAAGGAGCTGC
BKK15570 (cysH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCTTCTTTCCTCCTTT,  downstream forward: _UP4_CTGCATGAATAAGGAGCTGC
Labs working on this gene/protein
[[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France
References
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51EB09C122A7166D662AE72842A44EC5FBA03134

Page visits: 6369

Time of last update: 2025-04-10 01:28:22

Author of last update: Melvin.boenninger