cah

cah
168

cephalosporin C deacetylase

Locus
BSU_03180
Molecular weight
35.65 kDa
Isoelectric point
5.42
Protein length
318 aaSequenceBlast
Gene length
957 bpSequenceBlast
Function
resistance to cephalosporin C
Product
cephalosporin C deacetylase)
Essential
no
E.C.
3.1.1.41
Synonyms
cah
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3458 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
342,538  343,494
The protein
Catalyzed reaction/ biological activity
cephalosporin C + H2O --> acetate + deacetylcephalosporin C + H+ (according to UniProt)
Protein family
carbohydrate esterase 7 family (single member, according to UniProt)
Structure
1ODS (PDB) PubMed
P94388 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
cah
Description
PubMed
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cah

2025-04-10 02:41:00

ghost

63

46b1368ad1cd04bb9d87f7bbc3b755755eb00690

76F65BAC4D0FEBD2822136E5D7F9FD61492B947F

Genes
ycgK-cah-ycgL
Description
PubMed
Open in new tab

ycgK→ycgL

2025-04-05 05:16:12

Jstuelk

77

ab6bd4636d1fc5110090b1b0ce77491e89fa4b6d

B23BDB1B6636DD3E6BBB58391EB252FE951AC9F0

Biological materials
Mutant
BKE03180 (cah::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCCGATTATTCCCCTT,  downstream forward: _UP4_TGATAAATGTGAAAAGCCGC
BKK03180 (cah::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCCGATTATTCCCCTT,  downstream forward: _UP4_TGATAAATGTGAAAAGCCGC
References
Takimoto A, Yagi S, Mitsushima K High-level expression, purification, and some properties of a recombinant cephalosporin-C deacetylase. Journal of bioscience and bioengineering. 1999; 87(4):456-62. . PMID:16232499
Takimoto A, Takakura T, Tani H, Yagi S, Mitsushima K Batch production of deacetyl 7-aminocephalosporanic acid by immobilized cephalosporin-C deacetylase. Applied microbiology and biotechnology. 2004 Aug; 65(3):263-7. . PMID:15069587
Vincent F, Charnock SJ, Verschueren KH, Turkenburg JP, Scott DJ, Offen WA, Roberts S, Pell G, Gilbert HJ, Davies GJ, Brannigan JA Multifunctional xylooligosaccharide/cephalosporin C deacetylase revealed by the hexameric structure of the Bacillus subtilis enzyme at 1.9A resolution. Journal of molecular biology. 2003 Jul 11; 330(3):593-606. . PMID:12842474
Vincent F, Charnock SJ, Verschueren KH, Turkenburg JP, Scott DJ, Offen WA, Roberts S, Pell G, Gilbert HJ, Davies GJ, Brannigan JA Multifunctional xylooligosaccharide/cephalosporin C deacetylase revealed by the hexameric structure of the Bacillus subtilis enzyme at 1.9A resolution. Journal of molecular biology. 2003 Jul 11; 330(3):593-606. . PMID:12842474
Mitsushima K, Takimoto A, Sonoyama T, Yagi S Gene cloning, nucleotide sequence, and expression of a cephalosporin-C deacetylase from Bacillus subtilis. Applied and environmental microbiology. 1995 Jun; 61(6):2224-9. . PMID:7793942

6BCE78F623A40B519211E79AFA1AD898318BD817

Page visits: 4435

Time of last update: 2025-04-10 06:48:58

Author of last update: Melvin.boenninger