ahpF

ahpF
168

alkyl hydroperoxide reductase (large subunit) / NADH dehydrogenase

Locus
BSU_40100
Molecular weight
54.71 kDa
Isoelectric point
4.7
Protein length
509 aaSequenceBlast
Gene length
1530 bpSequenceBlast
Function
resistance against peroxide stress
Product
alkyl hydroperoxide reductase (large subunit) / NADH dehydrogenase
Essential
no
E.C.
1.6.99.3
Synonyms
ahpF, ndh
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3634 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,119,527  4,121,056
The protein
Catalyzed reaction/ biological activity
A + H+ + NADH --> AH2 + NAD+ (according to UniProt)
Protein family
class-II pyridine nucleotide-disulfide oxidoreductase family (with TrxB, according to UniProt)
Domains
Thioredoxin-like fold domain (aa 108-210) (according to InterPro)
Cofactors
FAD (according to UniProt)
Structure
1HYU (PDB) (from Salmonella typhimurium, 52% identity, 69% similarity) PubMed
P42974 (AlphaFold)
Modification
phosphorylation on (Ser-48 OR Ser-49) PubMed
phosphorylated on Arg-457 PubMed
Localization
cell membrane (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
ahpC-ahpF
Description
PubMed
Regulation
induced by H2O2 (PerR) PubMed
Regulatory mechanism
PerR: repression, PubMed, in perR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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ahpC→ahpF

2025-03-09 08:55:26

ghost

115

faf2593fbfc50585279e38e62b17a9a5b60cff9f

9798B313F3B383CB7131D3E1F8AD0DA2D1B1888C

Biological materials
Mutant
GP1730 ahpCF::mls trpC2 available in Jörg Stülke's lab
BKE40100 (ahpF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCAAGTACCAATTGAATGC,  downstream forward: _UP4_TAATATAAGAAATCCGCTAT
BKK40100 (ahpF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCAAGTACCAATTGAATGC,  downstream forward: _UP4_TAATATAAGAAATCCGCTAT
References
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8F23B575BC32EA63267C4321DD8E4BD546B11AF1

Page visits: 6526

Time of last update: 2025-04-15 04:09:40

Author of last update: Melvin.boenninger