BglA

• Description: 6-phospho-beta-glucosidase
  
  

Gene name	bglA
Synonyms
Essential	no
Product	6-phospho-beta-glucosidase)
Function	beta-glucoside utilization
Gene expression levels in SubtiExpress: bglA
MW, pI	54 kDa, 4.648
Gene length, protein length	1437 bp, 479 aa
Immediate neighbours	ahpF, yyzE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

The gene

Basic information

• Locus tag: BSU40110

Database entries

• BsubCyc: BSU40110

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H₂O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 1 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU40110

• Structure:

• UniProt: P42973

• KEGG entry: [3]

• E.C. number: 3.2.1.86

Expression and regulation

• Operon: yyzE-bglA (according to [4] DBTBS)

• Expression browser: bglA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 230 PubMed

• • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 362 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

B Setlow, R-M Cabrera-Martinez, P Setlow
Mechanism of the hydrolysis of 4-methylumbelliferyl-beta-D-glucoside by germinating and outgrowing spores of Bacillus species.
J Appl Microbiol: 2004, 96(6);1245-55
[PubMed:15139916] [WorldCat.org] [DOI] (P p)

Barbara Setlow, Arturo Cabrera-Hernandez, Rosa Martha Cabrera-Martinez, Peter Setlow
Identification of aryl-phospho-beta-D-glucosidases in Bacillus subtilis.
Arch Microbiol: 2004, 181(1);60-7
[PubMed:14652714] [WorldCat.org] [DOI] (P p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)

J Zhang, A Aronson
A Bacillus subtilis bglA gene encoding phospho-beta-glucosidase is inducible and closely linked to a NADH dehydrogenase-encoding gene.
Gene: 1994, 140(1);85-90
[PubMed:8125345] [WorldCat.org] [DOI] (P p)