ganA

ganA
168

beta-galactosidase

Locus
BSU_34130
Molecular weight
79.08 kDa
Isoelectric point
5.7
Protein length
687 aaSequenceBlast
Gene length
2064 bpSequenceBlast
Function
galactan utilization
Product
beta-galactosidase
Essential
no
E.C.
3.2.1.23
Synonyms
ganA, yvfN, lacA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1874 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,503,020 → 3,505,083
Phenotypes of a mutant
a ''ganS-ganP-ganQ-ganA-ganB'' mutant is impaired in the utilization of galactan PubMed
The protein
Catalyzed reaction/ biological activity
degradation of galactotriose to galactose PubMed
degradation of ß-1.4-galactobiose to galactose PubMed
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides (according to UniProt)
Protein family
glycosyl hydrolase 42 family (with YesZ, according to UniProt)
Structure
4OJY (PDB) (from Geobacillus stearothermophilus, 76% identity) PubMed
O07012 (AlphaFold)
Modification
phosphorylated on Arg-387 PubMed
Effectors of protein activity
inhibited by galactose PubMed
Expression and Regulation
Operons
Genes
ganS-ganP-ganQ-ganA-ganB
Description
PubMed
Regulation
induced by galactan (binding of galactobiose to GanR) PubMed
repressed by glucose (CcpA) PubMed
Regulatory mechanism
GanR: repression, PubMed, in ganR regulon
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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ganS→ganB

2025-04-09 00:22:07

Jstuelk

99

8098d66636fb8292b3a8cdd7bc3488e2a6cd4b3a

CC7DD5B518C2C1EF65E89AEC569B449F25EC1495

Biological materials
Mutant
GP1181 (aphA3) available in Jörg Stülke's lab
GP2147 (phleo) available in Jörg Stülke's lab
1A785 ( ganA::spec), PubMed, available at BGSC
1A786 ( ganA::spec), PubMed, available at BGSC
BKE34130 (ΔganA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACATTCTCCTCCTTGT,  downstream forward: _UP4_TAGGCTGATGCTCCGCTCGA
BKK34130 (ΔganA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACATTCTCCTCCTTGT,  downstream forward: _UP4_TAGGCTGATGCTCCGCTCGA
References
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DBBA549C2D71CF628E92AC7B659EA276E227D742

Page visits: 19248

Time of last update: 2025-04-10 01:56:26

Author of last update: Melvin.boenninger