MgtE

• Description: primary magnesium transporter
  
  

Gene name	mgtE
Synonyms	ykoK
Essential	no
Product	primary magnesium transporter
Function	magnesium uptake
Gene expression levels in SubtiExpress: mgtE
Metabolic function and regulation of this protein in SubtiPathways: MgtE
MW, pI	50 kDa, 4.609
Gene length, protein length	1353 bp, 451 aa
Immediate neighbours	ykzO, tnrA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), membrane proteins

This gene is a member of the following regulons

M-box

The gene

Basic information

• Locus tag: BSU13300

Phenotypes of a mutant

• a mgtE mutant has a growth defect on rich media, this can be suppressed by the addition of 25 mM Mg²⁺, moreover the mutant grows well on minimal medim in the presence of citrate PubMed
• a mgtE yfjQ mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg²⁺, moreover the mutant grows well on minimal medim in the presence of citrate PubMed

Database entries

• BsubCyc: BSU13300

• DBTBS entry: no entry
• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: SLC41A transporter family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU13300

• Structure: 2QBZ (the mgtE riboswitch)

• UniProt: O34442

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: mgtE PubMed

• Sigma factor:

• Regulation: induced at low magnesium concentration (M-box) PubMed

• Regulatory mechanism: M-box: Mg(2+)-responsive riboswitch PubMed

• Additional information:
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 259 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: