skfB

skfB
168

thioether bond forming radical SAM enzyme, catalyzes the first step in the maturation of Spore killing factor

Locus
BSU_01920
Molecular weight
45.59 kDa
Isoelectric point
4.93
Protein length
410 aaSequenceBlast
Gene length
1233 bpSequenceBlast
Function
maturation of Spore killing factor
Product
AdoMet radical sactisynthase
Essential
no
Synonyms
skfB, ybcP, ybcQ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0535 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, Spo0A regulon, AbrB regulon, PhoP regulon

Gene
Coordinates
214,175 → 215,407
The protein
Catalyzed reaction/ biological activity
generates a thioether bond in SkfA, which links the cysteine residue Cys4 with the α-carbon of the methionine residue Met12 PubMed
Protein family
Radical SAM superfamily (according to UniProt)
Cofactors
[4Fe-4S] and [2Fe-2S] clusters PubMed
Structure
6EFN (PDB) PubMed
O31423 (AlphaFold)
Expression and Regulation
Operons
Genes
skfA-skfB-skfC-skfE-skfF-skfG-skfH
Description
PubMed
Regulation
expressed under conditions that trigger sporulation (Spo0A) PubMed
Regulatory mechanism
Spo0A: activation, PubMed, in spo0A regulon
AbrB: repression, PubMed, in abrB regulon
PhoP: activation, PubMed, in phoP regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
Northern blotting during during phosphate limitation showed an intense 0.25 kb 'skfA'-specific transcript, and a weaker 6.5 kb 'skfA-skfB-skfC-skfE-skfF-skfG-skfH' transcript.
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skfA→skfH

2025-03-31 01:14:49

ghost

166

f96cb932f6aa46632dc613334e3526e3904b2534

E1A5B1DE7F5A4653FA0FC2E7FE7AAD3454DBFF28

Biological materials
Mutant
MGNA-C513 (ybcQ::erm), available at the NBRP B. subtilis, Japan
BKE01920 (ΔskfB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATAAAATCCCCCTTAAG,  downstream forward: _UP4_GAAGTCAAGGCAGGGATTCA
BKK01920 (ΔskfB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATAAAATCCCCCTTAAG,  downstream forward: _UP4_GAAGTCAAGGCAGGGATTCA
References
Reviews
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Research papers
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Time of last update: 2025-04-04 06:50:36

Author of last update: Jstuelk