qdoI

qdoI
168

Fe-containing quercetin 2,3-dioxygenase

Locus
BSU_39980
Molecular weight
37.43 kDa
Isoelectric point
5.56
Protein length
337 aaSequenceBlast
Gene length
1014 bpSequenceBlast
Function
resistance to plant product quercetin
Product
Fe-containing quercetin 2,3-dioxygenase
Essential
no
Synonyms
qdoI, yxaG
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1917 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,106,245  4,107,258
The protein
Catalyzed reaction/ biological activity
oxidation of the flavonol quercetin with dioxygen, cleaving the central heterocyclic ring and releasing CO
O2 + quercetin --> 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO (according to UniProt)
Protein family
bicupin family
Domains
2 cupin 2 domains (aa 55 ... 110, aa 226 ... 281)
Cofactors
Mn(II) (preferred), but also active with Fe(II) snd Co(II) PubMed
Structure
1Y3T (PDB) PubMed
2H0V (PDB)
P42106 (AlphaFold)
Expression and Regulation
Operons
Genes
qdoI-yxaH
Description
PubMed
Regulation
induced by flavonoids such as quercetin (QdoR, LmrA) PubMed
Regulatory mechanism
LmrA: repression, PubMed, in lmrA regulon
QdoR: repression, PubMed, in qdoR regulon
Additional information
major regulator: QdoR PubMed
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qdoI→yxaH

2025-03-12 23:00:43

ghost

71

c14e762fec9f4cbec17fdbace8ff20295b9c49d0

C80841584C27E4DC50FE3CA20B59B16E53149C1B

Biological materials
Mutant
MGNA-B684 (yxaG::erm), available at the NBRP B. subtilis, Japan
BKE39980 (qdoI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTATCGCTTCCTCCAA,  downstream forward: _UP4_TAAAGATGACAAGATTGTAA
BKK39980 (qdoI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTATCGCTTCCTCCAA,  downstream forward: _UP4_TAAAGATGACAAGATTGTAA
References
Reviews
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Original Publications
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6A68EF7BF16FC2F2C7E068F52901EF2BFD6EBF8A

Page visits: 3116

Time of last update: 2025-04-06 01:22:13

Author of last update: Melvin.boenninger