ntdA

ntdA
168

pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:glutamate aminotransferase

Locus
BSU_10550
Molecular weight
49.98 kDa
Isoelectric point
5.97
Protein length
441 aaSequenceBlast
Gene length
1326 bpSequenceBlast
Function
synthesis of the antibiotic kanosamine
Product
pyridoxal phosphate-dependent
Essential
no
Synonyms
ntdA, yhjL
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0399 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,128,286  1,129,611
The protein
Catalyzed reaction/ biological activity
3-oxo-d-glucose-6-phosphate + glutamate --> kanosamine-6-phosphate PubMed
Protein family
DegT/DnrJ/EryC1 family (with EpsN and SpsC, according to UniProt)
Cofactors
pyridoxalphosphate PubMed
Structure
4K2I (PDB) PubMed
O07566 (AlphaFold)
Expression and Regulation
Operons
Genes
ntdA-ntdB-ntdC-glcP
Description
PubMed
Regulation
induced by 3,3'-neotrehalosadiamine and kanosamine (NtdR) PubMed
Regulatory mechanism
NtdR: activation , in ntdR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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ntdA→glcP

2025-03-16 05:33:23

Jstuelk

109

41B772604ED3B8BA7F5F0AFF19B989A8E4F9FDC1

2A657E0F29C43EAF1C8F99F810D2B0756ABA88B3

Biological materials
Mutant
MGNA-A725 (yhjL::erm), available at the NBRP B. subtilis, Japan
BKE10550 (ntdA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAACAGTACCTCCAATG,  downstream forward: _UP4_TTTCATGTTATTAAGCAAGA
BKK10550 (ntdA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAACAGTACCTCCAATG,  downstream forward: _UP4_TTTCATGTTATTAAGCAAGA
References
Reviews
Inaoka T, Ochi K Activation of dormant secondary metabolism neotrehalosadiamine synthesis by an RNA polymerase mutation in Bacillus subtilis. Bioscience, biotechnology, and biochemistry. 2011; 75(4):618-23. . PMID:21512256
Original Publications
Vetter ND, Palmer DRJSubstrate Substitution in Kanosamine Biosynthesis Using Phosphonates and Phosphite Rescue.Biochemistry. 2021 Jun 7; . PMID: 34096710
Saito N, Nguyen HM, Inaoka TImpact of activation of neotrehalosadiamine/kanosamine biosynthetic pathway on the metabolism of Bacillus subtilis.Journal of bacteriology. 2021 Feb 22; . PMID: 33619155
Vetter ND, Jagdhane RC, Richter BJ, Palmer DRJCarbocyclic Substrate Analogues Reveal Kanosamine Biosynthesis Begins with the α-Anomer of Glucose 6-Phosphate.ACS chemical biology. 2020 Aug 21; 15(8):2205-2211. PMID: 32786294
van Straaten KE, Ko JB, Jagdhane R, Anjum S, Palmer DR, Sanders DA The structure of NtdA, a sugar aminotransferase involved in the kanosamine biosynthetic pathway in Bacillus subtilis, reveals a new subclass of aminotransferases. The Journal of biological chemistry. 2013 Nov 22; 288(47):34121-30. doi:10.1074/jbc.M113.500637. PMID:24097983
Vetter ND, Langill DM, Anjum S, Boisvert-Martel J, Jagdhane RC, Omene E, Zheng H, van Straaten KE, Asiamah I, Krol ES, Sanders DA, Palmer DR A previously unrecognized kanosamine biosynthesis pathway in Bacillus subtilis. Journal of the American Chemical Society. 2013 Apr 24; 135(16):5970-3. doi:10.1021/ja4010255. PMID:23586652
van Straaten KE, Langill DM, Palmer DR, Sanders DA Purification, crystallization and preliminary X-ray analysis of NtdA, a putative pyridoxal phosphate-dependent aminotransferase from Bacillus subtilis. Acta crystallographica. Section F, Structural biology and crystallization communications. 2009 Apr 01; 65(Pt 4):426-9. doi:10.1107/S1744309109009038. PMID:19342798
Inaoka T, Satomura T, Fujita Y, Ochi K Novel gene regulation mediated by overproduction of secondary metabolite neotrehalosadiamine in Bacillus subtilis. FEMS microbiology letters. 2009 Feb; 291(2):151-6. doi:10.1111/j.1574-6968.2008.01450.x. PMID:19087206
Inaoka T, Ochi K Glucose uptake pathway-specific regulation of synthesis of neotrehalosadiamine, a novel autoinducer produced in Bacillus subtilis. Journal of bacteriology. 2007 Jan; 189(1):65-75. . PMID:17056753
Inaoka T, Takahashi K, Yada H, Yoshida M, Ochi K RNA polymerase mutation activates the production of a dormant antibiotic 3,3'-neotrehalosadiamine via an autoinduction mechanism in Bacillus subtilis. The Journal of biological chemistry. 2004 Jan 30; 279(5):3885-92. . PMID:14612444

2F850E2DFA6287DB3890243803E06FDE7ACDB10E

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Time of last update: 2025-04-06 01:59:46

Author of last update: Jstuelk