gamP

gamP
168

glucosamine-specific permease of the phosphotransferase system, EIICBA of the PTS

Locus
BSU_02350
Molecular weight
67.97 kDa
Isoelectric point
5.47
Protein length
631 aaSequenceBlast
Gene length
1896 bpSequenceBlast
Function
glucosamine uptake and phosphorylation
Product
glucosamine-specific PTS, EIICBA
Essential
no
E.C.
2.7.1.69
Synonyms
gamP, ybfS, yzfA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2190 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
254,907  256,802
Phenotypes of a mutant
delayed growth with glucosamine as the single carbon source PubMed
The protein
Catalyzed reaction/ biological activity
protein EIIA Npi-phospho-L-histidine + protein EIIB --> protein EIIA + protein EIIB Npi-phospho-L-histidine/cysteine (according to UniProt)
Protein family
PTS permease, glucose family PubMed
Domains
PTS EIIA domain type-1 (aa 515-619) (according to UniProt)
PTS EIIB domain type-1 (aa 397-478) (according to UniProt)
PTS EIIC domain type-1 (aa 1-382) (according to UniProt)
Structure
6BVG (PDB) (EIIC domain from B. cereus, corresponds to aa 5 ... 380), 32.5% identity PubMed
3BP3 (PDB) (EIIB domain from E. coli, corresponds to aa 403 ... 467), 56.9% identity PubMed
1AX3 (PDB) (EIIA domain of PtsG, corresponds to aa 489 ... 622), 55.6% identity PubMed
P39816 (AlphaFold)
Paralogous protein(s)
NagP, PtsG, MalP
Localization
membrane PubMed
Expression and Regulation
Operons
Genes
gamA-gamP
Description
PubMed
Regulation
induced by glucosamine (GamR) PubMed
Regulatory mechanism
GamR: repression, PubMed, in gamR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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gamA→gamP

2025-03-09 02:38:53

ghost

66

4fffcf05befb972653171bbbe342dd9b2c49f8b3

1347DE6686FBBF7ED2CBD5C47BA6DFFC67338C93

Biological materials
Mutant
MGNA-B937 (ybfS::erm), available at the NBRP B. subtilis, Japan
QB6098 (aphA3), available in Jörg Stülke's lab
BKE02350 (gamP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACAGTCTCCTTTTATT,  downstream forward: _UP4_TAAAAAAGTCCCCCCTGCTG
BKK02350 (gamP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACAGTCTCCTTTTATT,  downstream forward: _UP4_TAAAAAAGTCCCCCCTGCTG
References
Reviews
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Original Publications
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4DF1740D4DFB9FD25E77C14D28AEA01707776099

Page visits: 5640

Time of last update: 2025-04-06 09:14:21

Author of last update: Jstuelk