murAA

murAA
168

UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Locus
BSU_36760
Molecular weight
46.54 kDa
Isoelectric point
5.45
Protein length
436 aaSequenceBlast
Gene length
1311 bpSequenceBlast
Function
peptidoglycan precursor biosynthesis
Product
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
Essential
yes
E.C.
2.5.1.7
Synonyms
murAA, murA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0766 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,777,949 → 3,779,259
Phenotypes of a mutant
essential PubMed
The protein
Catalyzed reaction/ biological activity
phosphoenolpyruvate + UDP-N-acetyl-α-D-glucosamine --> phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-α-D-glucosamine (according to UniProt)
Protein family
EPSP synthase family (with AroE and MurAB, according to UniProt)
Structure
3SG1 (PDB) (from B. anthracis, 79% identity, 94% similarity)
P70965 (AlphaFold)
Paralogous protein(s)
MurAB
Localization
cytoplasm (according to Swiss-Prot)
division septa PubMed
Additional information
MurAA is subject to ClpC-ClpP-dependent degradation upon glucose starvation  PubMed
Expression and Regulation
Operons
Genes
ywzB-spoIIT-murAA
Description
PubMed
Regulation
expressed early during sporulation in the forespore (SigF) PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigF: sigma factor, PubMed, in sigF regulon
Open in new tab

ywzB→murAA

2025-04-04 08:09:41

Bzhu

89

348b92db57f702141368550bd6f8ee854f499e36

A3D075B597018E5E660B1BB16E5A4B840E277744

Genes
murAA
Description
PubMed
Regulation
expressed early during sporulation in the forespore (SigF) PubMed
Open in new tab

murAA

2025-03-16 04:27:24

Bzhu

113

ab428b098c444d6051864b940c5bd49c48b8adce

E9A78A0A5F737601805178857AA7B293094805B4

Genes
spoIIT-murAA
Description
PubMed
Regulation
expressed early during sporulation in the forespore (SigF) PubMed
Open in new tab

spoIIT→murAA

2025-04-03 06:32:13

Bzhu

96

8686823a2584a3b9acd44b2f60efbd7dabdbf1a7

EC335D8C98E1864C25DE56486949E2C94D39B0B9

Additional information
the mRNA is cleaved by RNase III PubMed
Biological materials
Expression vectors
pRSETA-murAA, Expression of His(6)-MurAA, avalable in Ulf Gerth's lab PubMed
pGP2597: (IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172), available in Jörg Stülke's lab
Antibody
avalable in Ulf Gerth's lab PubMed
References
Reviews
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Original Publications
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DA763F7C2192655C590752C2A285ADF902B11BCA

Page visits: 5396

Time of last update: 2025-04-10 09:38:31

Author of last update: Jstuelk