Difference between revisions of "CitG"
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* '''Additional information:''' | * '''Additional information:''' | ||
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}} | ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}} | ||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 2427 {{PubMed|24696501}} | ||
+ | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 9880 {{PubMed|24696501}} | ||
=Biological materials = | =Biological materials = |
Revision as of 09:49, 17 April 2014
- Description: fumarase
Gene name | citG |
Synonyms | |
Essential | no |
Product | fumarate hydratase |
Function | TCA cycle |
Gene expression levels in SubtiExpress: citG | |
Interactions involving this protein in SubtInteract: CitG | |
Metabolic function and regulation of this protein in SubtiPathways: citG | |
MW, pI | 50 kDa, 5.475 |
Gene length, protein length | 1386 bp, 462 aa |
Immediate neighbours | yuxN, yvzF |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
carbon core metabolism, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU33040
Phenotypes of a mutant
Database entries
- BsubCyc: BSU33040
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-malate = fumarate + H2O (according to Swiss-Prot)
- Protein family: Fumarase subfamily (according to Swiss-Prot)
- Paralogous protein(s): AnsB
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU33040
- UniProt: P07343
- KEGG entry: [3]
- E.C. number: 4.2.1.2
Additional information
Expression and regulation
- Regulation: constitutive
- Regulatory mechanism:
- Additional information:
- belongs to the 100 most abundant proteins PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 2427 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 9880 PubMed
Biological materials
- Mutant:
- GP718 (spec), available in Jörg Stülke's lab
- Expression vector:
- pGP1122 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab)
- lacZ fusion:
- pGP387 (in pAC7), available in Jörg Stülke's lab
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
- FLAG-tag construct:
- GP1132 (spc, based on pGP1331), available in Jörg Stülke's lab
Labs working on this gene/protein
Your additional remarks
References