TyrS

• Description: tyrosyl-tRNA synthetase (major)
  
  

Gene name	tyrS
Synonyms
Essential	yes PubMed
Product	tyrosyl-tRNA synthetase (major)
Function	translation
Gene expression levels in SubtiExpress: tyrS
Metabolic function and regulation of this protein in SubtiPathways: tyrS
MW, pI	47 kDa, 5.213
Gene length, protein length	1266 bp, 422 aa
Immediate neighbours	rpsD, ytzK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, most abundant proteins

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU29670

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU29670

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU29670

• Structure:
  • 1JH3 (C-terminal domain, Geobacillus stearothermophilus)
  • 3TS1 (complex with tyrosyl adenylate intermediate, Geobacillus stearothermophilus)
  • 1N53 (the T-box antiterminator RNA) PubMed

• UniProt: P22326

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: tyrS PubMed

• Expression browser: tyrS PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by tyrosine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 848 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 3406 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

M Pelchat, J Lapointe
Aminoacyl-tRNA synthetase genes of Bacillus subtilis: organization and regulation.
Biochem Cell Biol: 1999, 77(4);343-7
[PubMed:10546897] [WorldCat.org] (P p)