pbpC

pbpC
168

transpeptidase, penicillin-binding protein 3

Locus
BSU_04140
Molecular weight
74.23 kDa
Isoelectric point
6.2
Protein length
668 aaSequenceBlast
Gene length
2007 bpSequenceBlast
Function
unknown
Product
class B penicillin-binding protein 3
Essential
no
Synonyms
pbpC, ycsM, yzsA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0768 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
463,934  465,940
Phenotypes of a mutant
increased sensitivity to several beta-lactams PubMed
The protein
Catalyzed reaction/ biological activity
cross-linking of glycan chains PubMed
Protein family
transpeptidase family (according to UniProt)
Structure
5E31 (PDB) (from Enterococcus faecium, 40% identity)
P42971 (AlphaFold)
Localization
mid cell localization (divisome), this depends on FtsZ and PBP2A PubMed
extracellular (signal peptide) PubMed
during vegetative growth: distinct foci and bands at cell periphery PubMed
localizes as patches in single cells and concentrates as a ring at the septum and the division site during the cell growth cycle PubMed
Expression and Regulation
Operons
Genes
pbpC-ycsN
Description
PubMed
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pbpC→ycsN

2025-04-06 20:52:16

Jstuelk

82

81abc64fde0e09e8c0327e08184eeaeaa1cd3255

4839481682B4D0CE488465BE6A1CEEDA34EC217C

Biological materials
Mutant
BKE04140 (pbpC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACTTTCCCCTGCCTTC,  downstream forward: _UP4_TAAAATGTCTTTTTAAAAGG
BKK04140 (pbpC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACTTTCCCCTGCCTTC,  downstream forward: _UP4_TAAAATGTCTTTTTAAAAGG
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab
GFP fusion
3105 trpC2 pbpC::pSG5045 (cat Pxyl-gfpa-pbpC1-768) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC
References
Reviews
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Original Publications
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5BBA2769A9C0EDB56625F008A55470C2E2885AA4

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Time of last update: 2025-04-13 00:10:21

Author of last update: Jstuelk