bioW

bioW
168

pimeloyl-CoA synthase (6-carboxyhexanoate-CoA ligase)

Locus
BSU_30240
Molecular weight
29.48 kDa
Isoelectric point
5.55
Protein length
259 aaSequenceBlast
Gene length
777 bpSequenceBlast
Function
biosynthesis of biotin
Product
pimeloyl-CoA synthase
Essential
no
E.C.
6.2.1.14
Synonyms
bioW
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG1424 (Galperin et al., 2021)

This gene is a member of the following regulons

BirA regulon

Gene
Coordinates
3,094,679 → 3,095,455
Phenotypes of a mutant
requires biotin for growth PubMed
The protein
Catalyzed reaction/ biological activity
ATP + CoA + heptanedioate --> AMP + diphosphate + heptanedioyl-CoA (according to UniProt)
Protein family
bioW family (single member, according to UniProt)
Structure
5FLG (PDB) PubMed
P53559 (AlphaFold)
Expression and Regulation
Operons
Genes
bioW-bioA-bioF-bioD-bioB-bioI-ytbQ
Description
PubMed
Regulation
repressed in the presence of biotin (BirA) PubMed
Regulatory mechanism
BirA: repression, PubMed, in birA regulon
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bioW→ytbQ

2025-04-03 13:40:07

ghost

82

8e74711c48d33750c45114afa6e28dd08cdf4ea5

F7255B320407AE19B7B38F8E863B5EF4A9BF3D52

Biological materials
Mutant
GP4743 (trpC2 ΔbioW::neo), available in Jörg Stülke's lab
BKE30240 (ΔbioW::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATTTCTCGCCCTTTCA,  downstream forward: _UP4_ATTTTAATAGAGTGGGAGGA
BKK30240 (ΔbioW::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATTTCTCGCCCTTTCA,  downstream forward: _UP4_ATTTTAATAGAGTGGGAGGA
LacZ fusion
pBP627 (in pAC7), available in Fabian Commichau's lab
References
Reviews
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Original Publications
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C40116089BAD3F9831F8C837DC4F2E045753ABDE

Page visits: 3488

Time of last update: 2025-04-15 09:29:36

Author of last update: Robert.warneke